economy we use unmodified concanavalin A (Sigma)

concanavalin a cona

G Protein Pathways, Part B: G Proteins and their Regulators

Cross-Linking Concanavalin A to SPR Chips
Concanavalin A is a tetrameric protein with four identical 28 kDa subunits. 29 The minimal structure exhibiting high affinity binding of carbohydrates is the dimeric form. The dimer form of ConA is an active stable structure, and the tetramer – dimer transition can be promoted by low pH or succinylation of the protein.30 For reasons of economy we use unmodified concanavalin A (Sigma). Because the dissociation of dimer from tetramer leads to a continual decline in the SPR baseline, our coupling procedures first promote the tetramer – dimer dissociation prior to linkage. To accomplish this, ConA is dissolved and stored at 4 ° in 100 mM sodium acetate, pH 4.8, prior to coupling. This also provides for optimal concentration of the ConA dimer by ionic attraction to the unactivated carboxyl groups. For limiting the ConA coupling the pH can be adjusted to 5.4 and still promote the dimer form.

concanavalin a
concanavalin a

However, extensive washing of the surface to allow complete tetramer dissociation is then necessary. Linkage of the ConA is accomplished by standard NHS / EDC amine coupling16 as described for streptavidin above, but with a modification in the running solution (Solution C, 50 mM MOPS, pH 7.5, 3 mM MgSO4, 150 mM NaCl with 10 μM CaCl2 and 10 μM MnCl2.

First, the surface is activated by injection of a mixture of 0.2 M EDC with 50 mM NHS in water at a flow rate of 5 μl / min, exposure time of 5–7 min. This is followed by injection of ConA at 0.1–0.3 mg / ml in 100 mM sodium acetate, pH 4.8, with an exposure time of 5–7 min. Activated carboxyls are then quenched by reaction with 1 M ethanolamine in water with an exposure time of 4 min. These procedures lead to the immobilization predominantly of dimeric ConA.

The immobilized ConA is quite stable, so that rhodopsin coupling does not have to proceed immediately. However, the coupling reactions are sufficiently reproducible and rapid that we find no advantage to prior preparation of ConA-modified surfaces. For experiments defining the independent binding of Gα or Gβγ described below, surfaces modified with 7000–12,000 RU of ConA have been utilized.

Under these conditions, the presence of immobilized tetrameric ConA is a significant factor. When high concentrations of ConA and / or aggressive NHS / EDC activation conditions are used, we routinely wash the ConA surface for 30–60 min with Solution C at a flow rate of 5 μl / min to allow for complete tetramer – dimer dissociation, prior to rhodopsin immobilization.

 

Concanavalin A

RM4157-25MG 1 unit
EUR 33.38
Description: Concanavalin A

Concanavalin A

inh-cona-2 2 x 100 mg
EUR 143.85

Concanavalin A

MBS3607807-25mg 25mg
EUR 200

Concanavalin A

MBS3607807-5x25mg 5x25mg
EUR 580

Concanavalin A

MBS5750377-100mg 100mg
EUR 175

Concanavalin A

MBS5750377-25mg 25mg
EUR 145

Concanavalin A

MBS5750377-50mg 50mg
EUR 150

Concanavalin A

MBS5750377-5x100mg 5x100mg
EUR 630

Concanavalin A IV

abx082153-25mg 25 mg
EUR 309.6

Concanavalin A IV

abx082153-100l 100 µl
EUR 162.5

Concanavalin A IV

abx082153-1ml 1 ml Ask for price

Concanavalin A IV

abx082153-200l 200 µl Ask for price

Concanavalin A (ConA)

05-0106-100 100 mg
EUR 91.2

Concanavalin A (ConA)

05-0106-1000 1 g
EUR 238.8

Concanavalin A (ConA)

05-0106-10000 10 g Ask for price

Concanavalin A (ConA)

05-0106-100000 100 g Ask for price

Concanavalin A (ConA)

05-0106-250 250 mg
EUR 105.6

Concanavalin A (ConA)

E16FXP010 1 mg
EUR 86.67
Description: Available in various conjugation types.

Concanavalin-A Antibody

MBS7152270-INQUIRE INQUIRE Ask for price

BioMagPlus Concanavalin A

BP531-10 10 ml
EUR 1399.73
Description: BioMagPlus Concanavalin A is magnetic particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule

BioMagPlus Concanavalin A

BP531-3 3 ml
EUR 621.95
Description: BioMagPlus Concanavalin A is magnetic particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule

Concanavalin A ELISA Kit

20-abx052181
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Concanavalin A ELISA Kit

ECP7827 96 Tests
EUR 479

Concanavalin A (Con A) Antibody

20-abx101152
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Concanavalin A agarose gel

EA-IP-014 2mL
EUR 242
Description: Primary Antibodies

Concanavalin A (ConA) Antibody

abx101152-100l 100 µl
EUR 287.5

Concanavalin A (ConA) Antibody

abx101152-1ml 1 ml
EUR 850

Concanavalin A (ConA) Antibody

abx101152-200l 200 µl
EUR 375

Anti- Concanavalin A Antibody

GWB-5B3EDC 1 ml Ask for price

Concanavalin A Lectin (Con A) - Cy3

21761096-1 1 mg
EUR 148.5

Concanavalin A Lectin (Con A) - Cy5

21761160-1 1 mg
EUR 142.41

Concanavalin A Magnetic Beads

K1308-1 1ml
EUR 44.8
Description: Protein & Immunology|Protein Purification and isolation

Concanavalin A Magnetic Beads

K1308-5 5x1ml
EUR 196.8
Description: Protein & Immunology|Protein Purification and isolation

Concanavalin A Magnetic beads

EA-IP-014M 1mL
EUR 159
Description: Primary Antibodies

Concanavalin A Lectin (Con A) - Pure

22070010-1 250 mg
EUR 111

Concanavalin A Lectin (Con A) - Pure

22070010-3 25 mg
EUR 72.06

Concanavalin A Lectin (Con A) - Pure

22070010-4 50 mg
EUR 104.25

Concanavalin A, CF®350 Conjugate

29015 5x1mg
EUR 189
Description: Lectin from Jack bean (Canavalia ensiformis)

Concanavalin A, CF®350 Conjugate

29015-1 PK
EUR 189

Concanavalin A, CF®594 Conjugate

29017 5x1mg
EUR 189
Description: Lectin from Jack bean (Canavalia ensiformis)

Concanavalin A, CF®594 Conjugate

29017-1 PK
EUR 189

Concanavalin A, CF®633 Conjugate

29018 5x1mg
EUR 189
Description: Lectin from Jack bean (Canavalia ensiformis)

Concanavalin A, CF®633 Conjugate

29018-1 PK
EUR 189

Concanavalin A, CF®680 Conjugate

29020-1 PK
EUR 224

Concanavalin A, CF®770 Conjugate

29058 5x1mg
EUR 224
Description: Lectin from Jack bean (Canavalia ensiformis)

Concanavalin A, CF®770 Conjugate

29058-1 PK
EUR 224

Concanavalin A, CF®750 Conjugate

29080 5x1mg
EUR 224
Description: Lectin from Jack bean (Canavalia ensiformis)

Concanavalin A, CF®750 Conjugate

29080-1 PK
EUR 224

Concanavalin A (ConA) CLIA Kit

20-abx190017
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests