economy we use unmodified concanavalin A (Sigma)
G Protein Pathways, Part B: G Proteins and their Regulators
Cross-Linking Concanavalin A to SPR Chips
Concanavalin A is a tetrameric protein with four identical 28 kDa subunits. 29 The minimal structure exhibiting high affinity binding of carbohydrates is the dimeric form. The dimer form of ConA is an active stable structure, and the tetramer – dimer transition can be promoted by low pH or succinylation of the protein.30 For reasons of economy we use unmodified concanavalin A (Sigma). Because the dissociation of dimer from tetramer leads to a continual decline in the SPR baseline, our coupling procedures first promote the tetramer – dimer dissociation prior to linkage. To accomplish this, ConA is dissolved and stored at 4 ° in 100 mM sodium acetate, pH 4.8, prior to coupling. This also provides for optimal concentration of the ConA dimer by ionic attraction to the unactivated carboxyl groups. For limiting the ConA coupling the pH can be adjusted to 5.4 and still promote the dimer form.
However, extensive washing of the surface to allow complete tetramer dissociation is then necessary. Linkage of the ConA is accomplished by standard NHS / EDC amine coupling16 as described for streptavidin above, but with a modification in the running solution (Solution C, 50 mM MOPS, pH 7.5, 3 mM MgSO4, 150 mM NaCl with 10 μM CaCl2 and 10 μM MnCl2.
First, the surface is activated by injection of a mixture of 0.2 M EDC with 50 mM NHS in water at a flow rate of 5 μl / min, exposure time of 5–7 min. This is followed by injection of ConA at 0.1–0.3 mg / ml in 100 mM sodium acetate, pH 4.8, with an exposure time of 5–7 min. Activated carboxyls are then quenched by reaction with 1 M ethanolamine in water with an exposure time of 4 min. These procedures lead to the immobilization predominantly of dimeric ConA.
The immobilized ConA is quite stable, so that rhodopsin coupling does not have to proceed immediately. However, the coupling reactions are sufficiently reproducible and rapid that we find no advantage to prior preparation of ConA-modified surfaces. For experiments defining the independent binding of Gα or Gβγ described below, surfaces modified with 7000–12,000 RU of ConA have been utilized.
Under these conditions, the presence of immobilized tetrameric ConA is a significant factor. When high concentrations of ConA and / or aggressive NHS / EDC activation conditions are used, we routinely wash the ConA surface for 30–60 min with Solution C at a flow rate of 5 μl / min to allow for complete tetramer – dimer dissociation, prior to rhodopsin immobilization.
 BioMagPlus Concanavalin A |
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| BP531-10 | Bangs Laboratories | 10 ml | EUR 1399.73 |
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Description: BioMagPlus Concanavalin A is magnetic particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule |
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BioMagPlus Concanavalin A |
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| BP531-3 | Bangs Laboratories | 3 ml | EUR 621.95 |
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Description: BioMagPlus Concanavalin A is magnetic particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule |
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 Concanavalin A ELISA Kit |
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| 20-abx052181 | Abbexa |
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 Antibody) Concanavalin A (Con A) Antibody |
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| 20-abx101152 | Abbexa |
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 Concanavalin A, CF350 conjugate |
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| 29015 | Biotium | 5x1mg | EUR 250.8 |
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Description: Minimum order quantity: 1 unit of 5x1mg |
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 Concanavalin A, CF594 conjugate |
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| 29017 | Biotium | 5x1mg | EUR 250.8 |
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Description: Minimum order quantity: 1 unit of 5x1mg |
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 Concanavalin A, CF633 conjugate |
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| 29018 | Biotium | 5x1mg | EUR 250.8 |
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Description: Minimum order quantity: 1 unit of 5x1mg |
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 Concanavalin A, CF680 conjugate |
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| 29020 | Biotium | 5x1mg | EUR 288 |
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Description: Minimum order quantity: 1 unit of 5x1mg |
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 Concanavalin A, CF770 conjugate |
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| 29058 | Biotium | 5x1mg | EUR 288 |
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Description: Minimum order quantity: 1 unit of 5x1mg |
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 Concanavalin A, CF750 conjugate |
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| 29080 | Biotium | 5x1mg | EUR 288 |
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Description: Minimum order quantity: 1 unit of 5x1mg |
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 Concanavalin A, CF488A conjugate |
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| 29016 | Biotium | 5x1mg | EUR 250.8 |
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Description: Minimum order quantity: 1 unit of 5x1mg |
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 Concanavalin A, CF640R conjugate |
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| 29019 | Biotium | 5x1mg | EUR 250.8 |
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Description: Minimum order quantity: 1 unit of 5x1mg |
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 Concanavalin A, CF405M conjugate |
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| 29074 | Biotium | 5x1mg | EUR 262.8 |
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Description: Minimum order quantity: 1 unit of 5x1mg |
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 Concanavalin A, CF405S conjugate |
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| 29075 | Biotium | 5x1mg | EUR 262.8 |
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Description: Minimum order quantity: 1 unit of 5x1mg |
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 CLIA Kit) Concanavalin A (ConA) CLIA Kit |
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| 20-abx190017 | Abbexa |
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 Rat Concanavalin A ELISA kit |
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| E02C0795-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Rat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Concanavalin A ELISA kit |
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| E02C0795-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Concanavalin A ELISA kit |
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| E02C0795-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Rat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 Dog Concanavalin A ELISA kit |
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| E08C0795-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Canine Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Concanavalin A ELISA kit |
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| E08C0795-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Canine Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Concanavalin A ELISA kit |
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| E08C0795-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Canine Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 Pig Concanavalin A ELISA kit |
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| E07C0795-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Porcine Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Concanavalin A ELISA kit |
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| E07C0795-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Porcine Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Concanavalin A ELISA kit |
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| E07C0795-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Porcine Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 ELISA Kit) Concanavalin A (ConA) ELISA Kit |
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| 20-abx150329 | Abbexa |
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 Goat Concanavalin A ELISA kit |
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| E06C0795-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Goat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Concanavalin A ELISA kit |
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| E06C0795-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Goat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Concanavalin A ELISA kit |
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| E06C0795-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Goat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 Human Concanavalin A ELISA kit |
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| E01C0795-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Human Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Concanavalin A ELISA kit |
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| E01C0795-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Human Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Concanavalin A ELISA kit |
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| E01C0795-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Human Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 Mouse Concanavalin A ELISA kit |
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| E03C0795-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Mouse Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Concanavalin A ELISA kit |
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| E03C0795-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Mouse Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Concanavalin A ELISA kit |
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| E03C0795-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Mouse Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 Rabbit Concanavalin A ELISA kit |
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| E04C0795-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Rabbit Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit Concanavalin A ELISA kit |
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| E04C0795-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rabbit Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit Concanavalin A ELISA kit |
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| E04C0795-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Rabbit Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 Monkey Concanavalin A ELISA kit |
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| E09C0795-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Monkey Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey Concanavalin A ELISA kit |
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| E09C0795-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Monkey Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey Concanavalin A ELISA kit |
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| E09C0795-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Monkey Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 Antibody Pair) Concanavalin A (ConA) Antibody Pair |
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| 20-abx370219 | Abbexa |
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 Antibody (Biotin)) Concanavalin A (ConA) Antibody (Biotin) |
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| 20-abx271751 | Abbexa |
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 ELISA Kit) Cat Concanavalin A (ConA) ELISA Kit |
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| YLA0097CT-48T | Shanghai YL Biotech | 48T | EUR 547.5 |
Cat Concanavalin A (ConA) ELISA Kit |
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| YLA0097CT-96T | Shanghai YL Biotech | 96T | EUR 637.5 |
 Human concanavalin A,ConA ELISA Kit |
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| 201-12-1781 | SunredBio | 96 tests | EUR 528 |
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Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
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Human concanavalin A,ConA ELISA Kit |
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| CN-04253H1 | ChemNorm | 96T | EUR 526.8 |
Human concanavalin A,ConA ELISA Kit |
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| CN-04253H2 | ChemNorm | 48T | EUR 348 |
ELISA Kit) Human concanavalin A(ConA)ELISA Kit |
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| GA-E1797HM-48T | GenAsia Biotech | 48T | EUR 346.8 |
Human concanavalin A(ConA)ELISA Kit |
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| GA-E1797HM-96T | GenAsia Biotech | 96T | EUR 559.2 |