MethylFlash Methylated DNA

Methyl fresh

Safe and Convenient

All the needed reagents, including negative controls and positive controls, for quantification of global DNA methylation are conveniently packaged in the kit. The direct colorimetric quantification of DNA samples replaces obsolete or inferior methods and eliminates the need for DNA digestion / denaturation, radioactivity, extraction, or chromatography.

Highly Sensitive and Specific
The novel procedure and proprietary kit compositions allow for accurate quantification of methylated DNA to be achieved with high sensitivity and specificity. The detection limit of the input DNA can be as low as 0.2 ng of methylated DNA.

Product Overview
We recommend using a newer version of this product: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in an ELISA-like microplate-based format. Compared to LUMA or LINE-1, Alu, and LTR-based assays, MethylFlash  technology directly quantifies current global DNA methylation.

This kit is also specifically optimized for paired use with the MethylFlash Hydroxymethylated DNA Quantification Kit (Colorimetric) for simultaneously quantifying both methylated DNA and hydroxymethylated DNA.

Product Features

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) is a further refinement of our previous Methylamp ™ global DNA methylation quantification kits by simplifying the workflow and improving the consistency of results. It uses an innovative method to enable background signals to be extremely low, eliminating the plate drying and blocking steps. Compared to chromatography-based methods such as HPLC or mass spectrometry, this product provides a cost-effective way to accurately measure levels of 5-methylcytosine. The kit has the following advantages and features:

methylflash methylated dna5-mc quantification kit
methylflash methylated dna5-mc quantification kit

Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours.
Innovative kit composition enables background signals to be extremely low, which eliminates the need for plate blocking and allows the assay to be simple, accurate, reliable, and consistent.
High sensitivity, of which the detection limit can be as low as 0.2 ng of methylated DNA and accepts as low as 50 ng of input genomic DNA.
Optimized antibody and enhancer solutions allow high specificity to 5mC, with no cross-reactivity to unmethylated cytosine and no or negligible cross-reactivity to hydroxymethylcytosine within the indicated concentration range of the sample DNA.
Universal positive and negative controls are included, which are suitable for quantifying methylated DNA from any species.
Strip-well microplate format makes the assay flexible: manual or high throughput analysis.

Principle & Procedure

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) contains all reagents necessary for the quantification of global DNA methylation. In this assay, DNA is bound to strip wells that are specifically treated to have a high DNA affinity. The methylated fraction of DNA is detected using capture and detection antibodies and then quantified through an ELISA-like reaction by reading the absorbance in a microplate spectrophotometer at 450 nm. The amount of methylated DNA is proportional to the OD intensity measured, which can be calculated with the kit’s included formulas for relative methylation status of two different DNA samples or absolute quantification of 5-methylcytosine (5mC) using a standard curve.

Easy, Fast, and Flexible

The entire colorimetric assay has easy-to-follow steps for convenience and speed, which can be completed in 4 hours. The strip-well microplate format allows for a flexible assay in manual or high throughput analysis. Universal positive and negative controls are included with the kit for quantifying methylated DNA from any species such as mammals, plants, fungi, bacteria, and viruses.

 

 

MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric) 

P-1041
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  • 96 Assays
  • 48 Assays
  • 96 Assays

MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)

P-1032
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  • 96 reactions
  • 48 reactions
  • 96 reactions

Rat Methylated DNA Control

D6434149 5 ug Ask for price

Methylamp Universal Methylated DNA Kit 

P-1011
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  • 20 Modifications
  • 10 Modifications
  • 20 Modifications

Human Methylated DNA Control

D6255815 5 ug
EUR 147

Mouse Methylated DNA Control

D6334149 5 ug Ask for price

Methylamp Methylated DNA Capture (MeDIP) Kit 

P-1015
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  • 48 Reactions
  • 24 Reactions
  • 48 Reactions

Monkey (Rhesus) Methylated DNA Control

D6534149 5 ug Ask for price

Methylamp Universal Methylated DNA Preparation Kit

P-1019 40 µg
EUR 416.7

MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Colorimetric) 

P-1039
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  • 96 Assays
  • 48 Assays
  • 96 Assays

MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Fluorometric) 

P-1040
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  • 96 Assays
  • 48 Assays
  • 96 Assays

Monkey (Cynomolgus) Methylated DNA Control

D6534149-Cy 5 ug Ask for price

SuperSense Methylated DNA Quantification Kit

P-1021
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  • 96 Assays
  • 48 Assays
  • 96 Assays

EpiQuik Methylated DNA Immunoprecipitation Kit 

P-2019
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  • 48 Reactions
  • 24 Reactions
  • 48 Reactions

Fully CpG Methylated Human Genomic DNA

X10000 10 µg/100 µl Ask for price

MethylFlash Urine N6-methyladenosine (m6A) Quantification Kit (Colorimetric)

P-9015
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  • 96 Assays
  • 48 Assays
  • 96 Assays

Methylated-DNA--protein-cysteine methyltransferase

AP84971 1mg
EUR 2640

Methylated-DNA--protein-cysteine methyltransferase

AP85149 1mg
EUR 2640

Methylated-DNA--protein-cysteine methyltransferase

AP85431 1mg
EUR 2640

Methylated-DNA--protein-cysteine methyltransferase

AP85584 1mg
EUR 2640

Methylated-DNA--protein-cysteine methyltransferase

AP86064 1mg
EUR 2640

EpiQuik Tissue Methylated DNA Immunoprecipitation Kit 

P-2020
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  • 48 Reactions
  • 24 Reactions
  • 48 Reactions

Methylated-DNA--protein-cysteine methyltransferase Antibody

20-abx109997
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Methylated-DNA--protein-cysteine methyltransferase Antibody

abx109997-100l 100 µl
EUR 162.5

Methylated & Low-Methylated DNA Matched Pair - Human Lymphocyte cell line

D6254874-PP 2x5 ug
EUR 404

Methylated-DNA--protein-cysteine methyltransferase (MGMT) Antibody

abx402558-100g 100 µg
EUR 287.5

Methylated-DNA--protein-cysteine methyltransferase (ogt) Antibody

abx343866-100l 100 µl
EUR 350

Methylated-DNA--protein-cysteine methyltransferase (ogt) Antibody

abx343866-50l 50 µl
EUR 250

Dog Methylated-DNA--protein-cysteine methyltransferase ELISA Kit

EK4202 96 tests
EUR 799

Methylated & Low-Methylated DNA Matched Pair - Human Peripheral Blood Leukocyte

D6234148-PP 2x5 ug
EUR 404

Methylated-DNA--protein-cysteine methyltransferase Polyclonal Antibody

42551 100μg
EUR 390

Methylated-DNA--protein-cysteine methyltransferase Polyclonal Antibody

42551-100ul 100ul
EUR 399.6

Human Methylated-DNA--protein-cysteine methyltransferase ELISA Kit

EK4201 96 tests
EUR 599

Nab1p (Methylated), Antibody

GWB-B907F5 0.1 mg Ask for price

Methylated Lysine Antibody

abx448445-100ug 100 ug
EUR 644.4

Methylated Lysine Antibody

abx448445-1096tests 10 × 96 tests Ask for price

Methylated Lysine Antibody

abx448445-596tests 5 × 96 tests Ask for price

Methylated Lysine Antibody

abx448445-96tests 96 tests
EUR 562.5

Dog MGMT/ Methylated-DNA--protein-cysteine methyltransferase ELISA Kit

E0070Do 1 Kit
EUR 630
Description: 6-O-methylguanine-DNA methyltransferase,O-6-methylguanine-DNA-alkyltransferase,MGMT

Rat Methylated-DNA--protein-cysteine methyltransferase (MGMT) ELISA Kit

RK14561 96T
EUR 280

Dog MGMT / Methylated-DNA--protein-cysteine methyltransferase ELISA Kit

E2027d 96T
EUR 900

Rat Mgmt / Methylated-DNA--protein-cysteine methyltransferase ELISA Kit

E2027r 96T
EUR 796

Human MGMT/ Methylated-DNA--protein-cysteine methyltransferase ELISA Kit

E1588Hu 1 Kit
EUR 515
Description: 6-O-methylguanine-DNA methyltransferase,O-6-methylguanine-DNA-alkyltransferase,MGMT

Human MGMT(Methylated-DNA--protein-cysteine methyltransferase) ELISA Kit

EH2056 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human MGMT(Methylated-DNA--protein-cysteine methyltransferase) ELISA Kit

EKF57429-48T 48T
EUR 396.9

Human MGMT(Methylated-DNA--protein-cysteine methyltransferase) ELISA Kit

EKF57429-5x96T 5x96T
EUR 2693.25