MethylFlash Methylated DNA

Methyl fresh

Safe and Convenient

All the needed reagents, including negative controls and positive controls, for quantification of global DNA methylation are conveniently packaged in the kit. The direct colorimetric quantification of DNA samples replaces obsolete or inferior methods and eliminates the need for DNA digestion / denaturation, radioactivity, extraction, or chromatography.

Highly Sensitive and Specific
The novel procedure and proprietary kit compositions allow for accurate quantification of methylated DNA to be achieved with high sensitivity and specificity. The detection limit of the input DNA can be as low as 0.2 ng of methylated DNA.

Product Overview
We recommend using a newer version of this product: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in an ELISA-like microplate-based format. Compared to LUMA or LINE-1, Alu, and LTR-based assays, MethylFlash  technology directly quantifies current global DNA methylation.

This kit is also specifically optimized for paired use with the MethylFlash Hydroxymethylated DNA Quantification Kit (Colorimetric) for simultaneously quantifying both methylated DNA and hydroxymethylated DNA.

Product Features

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) is a further refinement of our previous Methylamp ™ global DNA methylation quantification kits by simplifying the workflow and improving the consistency of results. It uses an innovative method to enable background signals to be extremely low, eliminating the plate drying and blocking steps. Compared to chromatography-based methods such as HPLC or mass spectrometry, this product provides a cost-effective way to accurately measure levels of 5-methylcytosine. The kit has the following advantages and features:

methylflash methylated dna5-mc quantification kit
methylflash methylated dna5-mc quantification kit

Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours.
Innovative kit composition enables background signals to be extremely low, which eliminates the need for plate blocking and allows the assay to be simple, accurate, reliable, and consistent.
High sensitivity, of which the detection limit can be as low as 0.2 ng of methylated DNA and accepts as low as 50 ng of input genomic DNA.
Optimized antibody and enhancer solutions allow high specificity to 5mC, with no cross-reactivity to unmethylated cytosine and no or negligible cross-reactivity to hydroxymethylcytosine within the indicated concentration range of the sample DNA.
Universal positive and negative controls are included, which are suitable for quantifying methylated DNA from any species.
Strip-well microplate format makes the assay flexible: manual or high throughput analysis.

Principle & Procedure

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) contains all reagents necessary for the quantification of global DNA methylation. In this assay, DNA is bound to strip wells that are specifically treated to have a high DNA affinity. The methylated fraction of DNA is detected using capture and detection antibodies and then quantified through an ELISA-like reaction by reading the absorbance in a microplate spectrophotometer at 450 nm. The amount of methylated DNA is proportional to the OD intensity measured, which can be calculated with the kit’s included formulas for relative methylation status of two different DNA samples or absolute quantification of 5-methylcytosine (5mC) using a standard curve.

Easy, Fast, and Flexible

The entire colorimetric assay has easy-to-follow steps for convenience and speed, which can be completed in 4 hours. The strip-well microplate format allows for a flexible assay in manual or high throughput analysis. Universal positive and negative controls are included with the kit for quantifying methylated DNA from any species such as mammals, plants, fungi, bacteria, and viruses.

 

 

MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric) 

P-1041
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  • 96 Assays
  • 48 Assays
  • 96 Assays

MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)

P-1032
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  • 96 reactions
  • 48 reactions
  • 96 reactions

MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Colorimetric) 

P-1039
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  • 96 Assays
  • 48 Assays
  • 96 Assays

MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Fluorometric) 

P-1040
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  • 96 Assays
  • 48 Assays
  • 96 Assays

MethylFlash Urine N6-methyladenosine (m6A) Quantification Kit (Colorimetric)

P-9015
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  • 96 Assays
  • 48 Assays
  • 96 Assays

Rat Methylated DNA Control

D6434149 5 ug Ask for price

Human Methylated DNA Control

D6255815 5 ug
EUR 147

Mouse Methylated DNA Control

D6334149 5 ug Ask for price

Methylamp Universal Methylated DNA Kit 

P-1011
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  • 20 Modifications
  • 10 Modifications
  • 20 Modifications

Methylamp Methylated DNA Capture (MeDIP) Kit 

P-1015
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  • 48 Reactions
  • 24 Reactions
  • 48 Reactions

Monkey (Rhesus) Methylated DNA Control

D6534149 5 ug Ask for price

Methylamp Universal Methylated DNA Preparation Kit

P-1019 40 µg
EUR 416.7

Monkey (Cynomolgus) Methylated DNA Control

D6534149-Cy 5 ug Ask for price

SuperSense Methylated DNA Quantification Kit

P-1021
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  • 96 Assays
  • 48 Assays
  • 96 Assays

EpiQuik Methylated DNA Immunoprecipitation Kit 

P-2019
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  • 48 Reactions
  • 24 Reactions
  • 48 Reactions

Fully CpG Methylated Human Genomic DNA

X10000 10 µg/100 µl Ask for price

Methylated-DNA--protein-cysteine methyltransferase

AP84971 1mg
EUR 2640

Methylated-DNA--protein-cysteine methyltransferase

AP85149 1mg
EUR 2640

Methylated-DNA--protein-cysteine methyltransferase

AP85431 1mg
EUR 2640

Methylated-DNA--protein-cysteine methyltransferase

AP85584 1mg
EUR 2640

Methylated-DNA--protein-cysteine methyltransferase

AP86064 1mg
EUR 2640

EpiQuik Tissue Methylated DNA Immunoprecipitation Kit 

P-2020
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  • 48 Reactions
  • 24 Reactions
  • 48 Reactions

Methylated-DNA--protein-cysteine methyltransferase Antibody

20-abx109997
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Methylated-DNA--protein-cysteine methyltransferase Antibody

abx109997-100l 100 µl
EUR 162.5

Methylated Lysine

AS15-3021 400 µl (250 µg/ml)
EUR 369

Methylated & Low-Methylated DNA Matched Pair - Human Lymphocyte cell line

D6254874-PP 2x5 ug
EUR 404

Methylated-DNA--protein-cysteine methyltransferase (MGMT) Antibody

abx402558-100g 100 µg
EUR 287.5

Methylated-DNA--protein-cysteine methyltransferase (ogt) Antibody

abx343866-100l 100 µl
EUR 350

Methylated-DNA--protein-cysteine methyltransferase (ogt) Antibody

abx343866-50l 50 µl
EUR 250

Methylated & Low-Methylated DNA Matched Pair - Human Peripheral Blood Leukocyte

D6234148-PP 2x5 ug
EUR 404

Recombinant human Methylated-DNA--protein-cysteine methyltransferase

MBS958443-002mg 0.02mg
EUR 255

Recombinant human Methylated-DNA--protein-cysteine methyltransferase

MBS958443-01mg 0.1mg
EUR 405

Recombinant human Methylated-DNA--protein-cysteine methyltransferase

MBS958443-1mg 1mg
EUR 1425

Recombinant human Methylated-DNA--protein-cysteine methyltransferase

MBS958443-5x1mg 5x1mg
EUR 6215

Pan Methylated Lysine

MBS9608307-01mL 0.1mL
EUR 260

Pan Methylated Lysine

MBS9608307-02mL 0.2mL
EUR 305

Pan Methylated Lysine

MBS9608307-5x02mL 5x0.2mL
EUR 1220

Dog Methylated-DNA--protein-cysteine methyltransferase ELISA Kit

EK4202 96 tests
EUR 799

Dog Methylated-DNA--protein-cysteine methyltransferase ELISA Kit

MBS2882756-10x96StripWells 10x96-Strip-Wells
EUR 4630

Dog Methylated-DNA--protein-cysteine methyltransferase ELISA Kit

MBS2882756-48StripWells 48-Strip-Wells
EUR 440

Dog Methylated-DNA--protein-cysteine methyltransferase ELISA Kit

MBS2882756-5x96StripWells 5x96-Strip-Wells
EUR 2480

Dog Methylated-DNA--protein-cysteine methyltransferase ELISA Kit

MBS2882756-96StripWells 96-Strip-Wells
EUR 580

Rat Methylated-DNA--protein-cysteine methyltransferase ELISA Kit

MBS289336-10x96StripWells 10x96-Strip-Wells
EUR 4080

Rat Methylated-DNA--protein-cysteine methyltransferase ELISA Kit

MBS289336-48StripWells 48-Strip-Wells
EUR 390

Rat Methylated-DNA--protein-cysteine methyltransferase ELISA Kit

MBS289336-5x96StripWells 5x96-Strip-Wells
EUR 2220

Rat Methylated-DNA--protein-cysteine methyltransferase ELISA Kit

MBS289336-96StripWells 96-Strip-Wells
EUR 520