MethylFlash Methylated DNA
Safe and Convenient
All the needed reagents, including negative controls and positive controls, for quantification of global DNA methylation are conveniently packaged in the kit. The direct colorimetric quantification of DNA samples replaces obsolete or inferior methods and eliminates the need for DNA digestion / denaturation, radioactivity, extraction, or chromatography.
Highly Sensitive and Specific
The novel procedure and proprietary kit compositions allow for accurate quantification of methylated DNA to be achieved with high sensitivity and specificity. The detection limit of the input DNA can be as low as 0.2 ng of methylated DNA.
Product Overview
We recommend using a newer version of this product: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)
The MethylFlash Methylated DNA Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in an ELISA-like microplate-based format. Compared to LUMA or LINE-1, Alu, and LTR-based assays, MethylFlash technology directly quantifies current global DNA methylation.
This kit is also specifically optimized for paired use with the MethylFlash Hydroxymethylated DNA Quantification Kit (Colorimetric) for simultaneously quantifying both methylated DNA and hydroxymethylated DNA.
Product Features
The MethylFlash Methylated DNA Quantification Kit (Colorimetric) is a further refinement of our previous Methylamp ™ global DNA methylation quantification kits by simplifying the workflow and improving the consistency of results. It uses an innovative method to enable background signals to be extremely low, eliminating the plate drying and blocking steps. Compared to chromatography-based methods such as HPLC or mass spectrometry, this product provides a cost-effective way to accurately measure levels of 5-methylcytosine. The kit has the following advantages and features:
Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours.
Innovative kit composition enables background signals to be extremely low, which eliminates the need for plate blocking and allows the assay to be simple, accurate, reliable, and consistent.
High sensitivity, of which the detection limit can be as low as 0.2 ng of methylated DNA and accepts as low as 50 ng of input genomic DNA.
Optimized antibody and enhancer solutions allow high specificity to 5mC, with no cross-reactivity to unmethylated cytosine and no or negligible cross-reactivity to hydroxymethylcytosine within the indicated concentration range of the sample DNA.
Universal positive and negative controls are included, which are suitable for quantifying methylated DNA from any species.
Strip-well microplate format makes the assay flexible: manual or high throughput analysis.
Principle & Procedure
The MethylFlash Methylated DNA Quantification Kit (Colorimetric) contains all reagents necessary for the quantification of global DNA methylation. In this assay, DNA is bound to strip wells that are specifically treated to have a high DNA affinity. The methylated fraction of DNA is detected using capture and detection antibodies and then quantified through an ELISA-like reaction by reading the absorbance in a microplate spectrophotometer at 450 nm. The amount of methylated DNA is proportional to the OD intensity measured, which can be calculated with the kit’s included formulas for relative methylation status of two different DNA samples or absolute quantification of 5-methylcytosine (5mC) using a standard curve.
Easy, Fast, and Flexible
The entire colorimetric assay has easy-to-follow steps for convenience and speed, which can be completed in 4 hours. The strip-well microplate format allows for a flexible assay in manual or high throughput analysis. Universal positive and negative controls are included with the kit for quantifying methylated DNA from any species such as mammals, plants, fungi, bacteria, and viruses.
 Human Methylated DNA Control |
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| D6255815 | Biochain | 5 ug | EUR 187.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 Mouse Methylated DNA Control |
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| D6334149 | Biochain | 5 ug | Ask for price |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 Rat Methylated DNA Control |
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| D6434149 | Biochain | 5 ug | Ask for price |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 ) MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric) |
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| P-1037-48 | EpiGentek | 48 Assays | EUR 463.1 |
MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric) |
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| P-1037-96 | EpiGentek | 96 Assays | EUR 775.5 |
MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric) |
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| P-1037 | EpiGentek |
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 Methylated DNA Control) Monkey (Rhesus) Methylated DNA Control |
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| D6534149 | Biochain | 5 ug | Ask for price |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 Methylated DNA Control) Monkey (Cynomolgus) Methylated DNA Control |
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| D6534149-Cy | Biochain | 5 ug | Ask for price |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 Methylamp Universal Methylated DNA Kit |
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| P-1011-1 | EpiGentek | 10 Modifications | EUR 292.6 |
Methylamp Universal Methylated DNA Kit |
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| P-1011-2 | EpiGentek | 20 Modifications | EUR 442.2 |
 SuperSense Methylated DNA Quantification Kit |
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| P-1021-48 | EpiGentek | 48 Assays | EUR 442.2 |
SuperSense Methylated DNA Quantification Kit |
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| P-1021-96 | EpiGentek | 96 Assays | EUR 760.1 |
 EpiQuik Methylated DNA Immunoprecipitation Kit |
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| P-2019-24 | EpiGentek | 24 Reactions | EUR 451 |
EpiQuik Methylated DNA Immunoprecipitation Kit |
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| P-2019-48 | EpiGentek | 48 Reactions | EUR 724.9 |
Methylamp Universal Methylated DNA Kit |
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| P-1011 | EpiGentek |
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SuperSense Methylated DNA Quantification Kit |
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| P-1021 | EpiGentek |
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EpiQuik Methylated DNA Immunoprecipitation Kit |
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| P-2019 | EpiGentek |
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 Methylated & Low-Methylated DNA Matching Pair - Human Peripheral Blood Leukocyte |
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| D6234148-PP | Biochain | 2x5 ug | EUR 411.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 Methylated & Low-Methylated DNA Matching Pair - Human Lymphocyte cell line |
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| D6254874-PP | Biochain | 2x5 ug | EUR 411.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 Methylated-DNA--protein-cysteine methyltransferase Antibody |
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| 20-abx109997 | Abbexa |
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 Kit ) Methylamp Methylated DNA Capture (MeDIP) Kit |
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| P-1015-24 | EpiGentek | 24 Reactions | EUR 424.6 |
Methylamp Methylated DNA Capture (MeDIP) Kit |
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| P-1015-48 | EpiGentek | 48 Reactions | EUR 712.8 |
 EpiQuik Tissue Methylated DNA Immunoprecipitation Kit |
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| P-2020-24 | EpiGentek | 24 Reactions | EUR 442.2 |
EpiQuik Tissue Methylated DNA Immunoprecipitation Kit |
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| P-2020-48 | EpiGentek | 48 Reactions | EUR 710.6 |
Methylamp Methylated DNA Capture (MeDIP) Kit |
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| P-1015 | EpiGentek |
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 Methylamp Universal Methylated DNA Preparation Kit |
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| P-1019 | EpiGentek | 40 µg | EUR 416.7 |
EpiQuik Tissue Methylated DNA Immunoprecipitation Kit |
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| P-2020 | EpiGentek |
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 Fully CpG Methylated Human Genomic DNA |
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| X10000 | EpiGentek | 10 µg/100 µl | Ask for price |
 100 BP DNA LADDER, 500UL PER KIT |
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| M-DNA-100BP | CORNING | 1/pk | EUR 87.6 |
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Description: Bioscience Mol Bio; DNA Ladder |
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 1 KB DNA LADDER, 500UL PER KIT |
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| M-DNA-1KB | CORNING | 1/pk | EUR 84 |
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Description: Bioscience Mol Bio; DNA Ladder |
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) MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric) |
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| P-1036-48 | EpiGentek | 48 Assays | EUR 424.6 |
MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric) |
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| P-1036-96 | EpiGentek | 96 Assays | EUR 738.1 |
MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric) |
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| P-1036 | EpiGentek |
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 Methylated-DNA--protein-cysteine methyltransferase Polyclonal Antibody |
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| 42551-100ul | SAB | 100ul | EUR 399.6 |
 DNA Quantification Kit (Colorimetric) ) MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric) |
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| P-1041-48 | EpiGentek | 48 Assays | EUR 442.2 |
MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric) |
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| P-1041-96 | EpiGentek | 96 Assays | EUR 760.1 |
MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric) |
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| P-1041 | EpiGentek |
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 Methylated-DNA--protein-cysteine methyltransferase Polyclonal Conjugated Antibody |
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| C42551 | SAB | 100ul | EUR 476.4 |
 ELISA Easy Kit (Colorimetric)) MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric) |
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| P-1030-48 | EpiGentek | 48 reactions | EUR 371.8 |
MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric) |
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| P-1030-96 | EpiGentek | 96 reactions | EUR 673.2 |
 ELISA Easy Kit (Colorimetric)) MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric) |
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| P-1032-48 | EpiGentek | 48 reactions | EUR 396 |
MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric) |
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| P-1032-96 | EpiGentek | 96 reactions | EUR 696.3 |
MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric) |
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| P-1032 | EpiGentek |
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MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric) |
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| P-1030 | EpiGentek |
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 Dog MGMT/ Methylated-DNA--protein-cysteine methyltransferase ELISA Kit |
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| E0070Do | Sunlong | 1 Kit | EUR 860.4 |
 ELISA Kit) Human MGMT(Methylated-DNA--protein-cysteine methyltransferase) ELISA Kit |
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| EH2056 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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