Safe and Convenient

All the needed reagents, including negative controls and positive controls, for quantification of global DNA methylation are conveniently packaged in the kit. The direct colorimetric quantification of DNA samples replaces obsolete or inferior methods and eliminates the need for DNA digestion / denaturation, radioactivity, extraction, or chromatography.

Highly Sensitive and Specific
The novel procedure and proprietary kit compositions allow for accurate quantification of methylated DNA to be achieved with high sensitivity and specificity. The detection limit of the input DNA can be as low as 0.2 ng of methylated DNA.

Product Overview
We recommend using a newer version of this product: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in an ELISA-like microplate-based format. Compared to LUMA or LINE-1, Alu, and LTR-based assays, MethylFlash  technology directly quantifies current global DNA methylation.

This kit is also specifically optimized for paired use with the MethylFlash Hydroxymethylated DNA Quantification Kit (Colorimetric) for simultaneously quantifying both methylated DNA and hydroxymethylated DNA.

Product Features

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) is a further refinement of our previous Methylamp ™ global DNA methylation quantification kits by simplifying the workflow and improving the consistency of results. It uses an innovative method to enable background signals to be extremely low, eliminating the plate drying and blocking steps. Compared to chromatography-based methods such as HPLC or mass spectrometry, this product provides a cost-effective way to accurately measure levels of 5-methylcytosine. The kit has the following advantages and features:

Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours.
Innovative kit composition enables background signals to be extremely low, which eliminates the need for plate blocking and allows the assay to be simple, accurate, reliable, and consistent.
High sensitivity, of which the detection limit can be as low as 0.2 ng of methylated DNA and accepts as low as 50 ng of input genomic DNA.
Optimized antibody and enhancer solutions allow high specificity to 5mC, with no cross-reactivity to unmethylated cytosine and no or negligible cross-reactivity to hydroxymethylcytosine within the indicated concentration range of the sample DNA.
Universal positive and negative controls are included, which are suitable for quantifying methylated DNA from any species.
Strip-well microplate format makes the assay flexible: manual or high throughput analysis.

Principle & Procedure

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) contains all reagents necessary for the quantification of global DNA methylation. In this assay, DNA is bound to strip wells that are specifically treated to have a high DNA affinity. The methylated fraction of DNA is detected using capture and detection antibodies and then quantified through an ELISA-like reaction by reading the absorbance in a microplate spectrophotometer at 450 nm. The amount of methylated DNA is proportional to the OD intensity measured, which can be calculated with the kit’s included formulas for relative methylation status of two different DNA samples or absolute quantification of 5-methylcytosine (5mC) using a standard curve.

Easy, Fast, and Flexible

The entire colorimetric assay has easy-to-follow steps for convenience and speed, which can be completed in 4 hours. The strip-well microplate format allows for a flexible assay in manual or high throughput analysis. Universal positive and negative controls are included with the kit for quantifying methylated DNA from any species such as mammals, plants, fungi, bacteria, and viruses.

MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric)
P-1041	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays

MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)
P-1032	EpiGentek	Ask for price Ask for price Ask for price	96 reactions 48 reactions 96 reactions

MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Colorimetric)
P-1039	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays

MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Fluorometric)
P-1040	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays

MethylFlash Urine N6-methyladenosine (m6A) Quantification Kit (Colorimetric)
P-9015	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays

Rat Methylated DNA Control
D6434149	Biochain	5 ug	Ask for price

Human Methylated DNA Control
D6255815	Biochain	5 ug	EUR 107.8

Mouse Methylated DNA Control
D6334149	Biochain	5 ug	Ask for price

Methylamp Universal Methylated DNA Kit
P-1011	EpiGentek	Ask for price Ask for price Ask for price	20 Modifications 10 Modifications 20 Modifications

Methylamp Methylated DNA Capture (MeDIP) Kit
P-1015	EpiGentek	Ask for price Ask for price Ask for price	48 Reactions 24 Reactions 48 Reactions

Monkey (Rhesus) Methylated DNA Control
D6534149	Biochain	5 ug	Ask for price

Methylamp Universal Methylated DNA Preparation Kit
P-1019	EpiGentek	40 µg	EUR 416.7

Monkey (Cynomolgus) Methylated DNA Control
D6534149-Cy	Biochain	5 ug	Ask for price

SuperSense Methylated DNA Quantification Kit
P-1021	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays

EpiQuik Methylated DNA Immunoprecipitation Kit
P-2019	EpiGentek	Ask for price Ask for price Ask for price	48 Reactions 24 Reactions 48 Reactions

Fully CpG Methylated Human Genomic DNA
X10000	EpiGentek	10 µg/100 µl	Ask for price

Methylated-DNA--protein-cysteine methyltransferase
AP86064	SAB	1mg	EUR 2640

Methylated-DNA--protein-cysteine methyltransferase
AP84971	SAB	1mg	EUR 2640

Methylated-DNA--protein-cysteine methyltransferase
AP85149	SAB	1mg	EUR 2640

Methylated-DNA--protein-cysteine methyltransferase
AP85431	SAB	1mg	EUR 2640

Methylated-DNA--protein-cysteine methyltransferase
AP85584	SAB	1mg	EUR 2640

EpiQuik Tissue Methylated DNA Immunoprecipitation Kit
P-2020	EpiGentek	Ask for price Ask for price Ask for price	48 Reactions 24 Reactions 48 Reactions

Methylated-DNA--protein-cysteine methyltransferase Antibody
abx109997-100l	Abbexa	100 µl	EUR 162.5

Methylated-DNA--protein-cysteine methyltransferase Antibody
20-abx109997	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	20 ug 50 ug 100 ug 200 ug 1 mg

Methylated Lysine
AS15-3021	Agrisera AB	400 µl (250 µg/ml)	EUR 369

Methylated & Low-Methylated DNA Matched Pair - Human Lymphocyte cell line
D6254874-PP	Biochain	2x5 ug	EUR 282.8

Methylated-DNA--protein-cysteine methyltransferase (ogt) Antibody
abx343866-100l	Abbexa	100 µl	EUR 350

Methylated-DNA--protein-cysteine methyltransferase (ogt) Antibody
abx343866-50l	Abbexa	50 µl	EUR 250

Methylated-DNA--protein-cysteine methyltransferase (MGMT) Antibody
abx402558-100g	Abbexa	100 µg	EUR 287.5

Methylated & Low-Methylated DNA Matched Pair - Human Peripheral Blood Leukocyte
D6234148-PP	Biochain	2x5 ug	EUR 282.8

Pan Methylated Lysine
MBS9608307-01mL	MyBiosource	0.1mL	EUR 260

Pan Methylated Lysine
MBS9608307-02mL	MyBiosource	0.2mL	EUR 305

Pan Methylated Lysine
MBS9608307-5x02mL	MyBiosource	5x0.2mL	EUR 1220

Recombinant human Methylated-DNA--protein-cysteine methyltransferase
MBS958443-002mg	MyBiosource	0.02mg	EUR 255

Recombinant human Methylated-DNA--protein-cysteine methyltransferase
MBS958443-01mg	MyBiosource	0.1mg	EUR 405

Recombinant human Methylated-DNA--protein-cysteine methyltransferase
MBS958443-1mg	MyBiosource	1mg	EUR 1425

Recombinant human Methylated-DNA--protein-cysteine methyltransferase
MBS958443-5x1mg	MyBiosource	5x1mg	EUR 6215

Dog Methylated-DNA--protein-cysteine methyltransferase ELISA Kit
EK4202	SAB	96 tests	EUR 799

Rat Methylated-DNA--protein-cysteine methyltransferase ELISA Kit
MBS289336-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 4080

Rat Methylated-DNA--protein-cysteine methyltransferase ELISA Kit
MBS289336-48StripWells	MyBiosource	48-Strip-Wells	EUR 390

Rat Methylated-DNA--protein-cysteine methyltransferase ELISA Kit
MBS289336-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 2220

Rat Methylated-DNA--protein-cysteine methyltransferase ELISA Kit
MBS289336-96StripWells	MyBiosource	96-Strip-Wells	EUR 520

Dog Methylated-DNA--protein-cysteine methyltransferase ELISA Kit
MBS2882756-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 4630

Dog Methylated-DNA--protein-cysteine methyltransferase ELISA Kit
MBS2882756-48StripWells	MyBiosource	48-Strip-Wells	EUR 440

Dog Methylated-DNA--protein-cysteine methyltransferase ELISA Kit
MBS2882756-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 2480

Dog Methylated-DNA--protein-cysteine methyltransferase ELISA Kit
MBS2882756-96StripWells	MyBiosource	96-Strip-Wells	EUR 580