Kash Biotech Express

Capabilities

• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Elimination of DNases and RNases when isolating DNA and RNA from tissues or cell traces
• Dedication of enzyme localization
• Enhancing cloning effectivity of PCR merchandise

Proteinase Okay – one among many usually used enzymes in molecular biology.

 

Thermo Scientific Proteinase Okay is a broad-range endolytic protease extensively used for digestion of proteins in nucleic acid preparations. It degrades proteins even all through the presence of detergents. Proteinase Okay cleaves peptide bonds on the carboxylic sides of aliphatic, fragrant, or hydrophobic amino acids. The Proteinase Okay is classed as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

Proteinase k kay (Lyophilized)

Secure at Room Temperature and Simple to Use

• Lively over pH differ 4.3–12.Zero in 0.5% SDS or 1% Triton X-100
• Retains >80% prepare at temperatures as lots as 60°C
• No resuspension or thawing ahead of use
• Cat.# MC5005, MC5008 provided at a highlight of 20mg/ml

Highlights

• Prepared-to-use reply
• Lively in a variety of response circumstances



Bear in mind

• The really useful working focus of Proteinase Okay is 0.05 to 1 mg/mL. The prepare of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to Four M urea
• Ca2+ protects Proteinase Okay in path of autolysis, will enhance the thermal stability, and has a regulatory perform for the substrate binding web site of Proteinase Okay
• Secure over a giant pH differ: 4.Zero to 12.5, optimum pH 7.5 to eight.0
• Optimum prepare at 50 to55°C
• Speedy denaturation of enzyme happens at temperatures above 65°C.

 

Proteinase Okay Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It’s a broad-spectrum endopeptidase with a really excessive particular prepare.

Recombinant Proteinase Okay enzyme is expressed in Pichia pastoris, and undergoes in depth purification to yield the simplest top of the range product.

Proteinase Okay Molecular Biology Grade (PCR Grade) is filled with life beneath a variety of response circumstances, together with elevated temperatures and the presence of SDS. In consequence, this enzyme is extensively utilizing for the digestion of proteins, together with DNases and RNases, all by way of nucleic acid preparations with out compromising the integrity of remoted DNA or RNA.

Selections

• Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
• Excessive prepare and distinctive purity.
• Lively at excessive temperatures (as lots as 56 °C) and denaturing circumstances (e.g. all through the presence of urea and/or SDS), which makes it best for digesting proteins in quite a lot of options.
• Secure over a giant pH differ: 4.0–12.5 (optimum pH 7.5–8.0).
• Decreased quantity of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
• Accessible as powder, lyophilized “cake” or liquid.

Capabilities:

• Extraction of DNA and RNA from fully completely completely different beginning offers.
• Purification of goal provides from contaminating proteins.
• Elimination of DNases and RNases all by way of nucleic acids isolation.

 

Proteinase K
GE0990-500MG	Glentham Life Sciences	500 mg	EUR 249

Proteinase K
P2180-010	GenDepot	100mg	EUR 182

Proteinase K
P2180-050	GenDepot	500mg	EUR 374

Proteinase K
P2180-100	GenDepot	1g	EUR 523

Proteinase K
BIO-37037	Bioline	100mg	Ask for price

Proteinase K
BIO-37039	Bioline	1000mg	Ask for price

Proteinase K
AR0056	BosterBio	5mL	EUR 101

Proteinase K
abx098140-1ml	Abbexa	1 ml	EUR 189

Proteinase K
abx082041-100mg	Abbexa	100 mg	EUR 258

Proteinase K
K1037-1	ApexBio	1 ml	EUR 67
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.

Proteinase K
K1037-5	ApexBio	5 ml	EUR 108
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.

Proteinase K
G029	ABM	1.0 ml	EUR 142

Proteinase K
Z5050002	Biochain	100 mg	EUR 171
Description: No genetic downstream application can do without the good old Proteinase K.

Proteinase K - 1g
PB0451	Bio Basic	1g	EUR 556.34

Proteinase K solution
GZ5904-100ML	Glentham Life Sciences	100 ml	EUR 907

Proteinase K solution
GZ5904-25ML	Glentham Life Sciences	25 ml	EUR 349

Proteinase K Solution
BIO-37084	Bioline	5ml @ 20mg/ml	Ask for price

Proteinase K Solution
BIO-37085	Bioline	5 x 5ml @ 20mg/ml	Ask for price

PROTEINASE K SOLUTION
501-PK	Viagen	5 ml	EUR 152
Description: Genomic PCR Grade Proteinase K Solution

10x Proteinase K
TG4039	TopoGen	0.5ml	EUR 134

Proteinase K protein
30R-2901	Fitzgerald	500 mg	EUR 425
Description: Purified recombinant Human Proteinase K protein

Proteinase K Antibody
6210-100	Biovision		EUR 294

Proteinase K Antibody
6210-30T	Biovision		EUR 146

Proteinase K, 20mg/ml
P2170-002	GenDepot	2X1ml	EUR 151

Proteinase K, 20mg/ml
P2170-005	GenDepot	5X1ml	EUR 199

PROTEINASE K SOLUTION 10ML
502-PK	Viagen	10 ml	EUR 239
Description: Genomic PCR Grade Proteinase K Solution

PROTEINASE K SOLUTION 25ML
503-PK	Viagen	25 ml	EUR 502
Description: Genomic PCR Grade Proteinase K Solution

Proteinase K (high conc.)
RP02503	Abclonal	100mg	Ask for price

Proteinase K from Tritirachium album
abx082503-100mg	Abbexa	100 mg	EUR 230

Proteinase K, Recombinant, Molecular Grade
9210-100	Biovision		EUR 120

Proteinase K, Recombinant, Molecular Grade
9210-10G	Biovision		EUR 2643

Proteinase K, Recombinant, Molecular Grade
9210-1G	Biovision		EUR 338

Proteinase K, Recombinant, Molecular Grade
9210-500	Biovision		EUR 262

Proteinase K, Recombinant, Molecular Grade
9210-50G	Biovision		Ask for price

Proteinase K, Recombinant, NGS Grade
9247-100	Biovision		EUR 175

Proteinase K, Recombinant, NGS Grade
9247-10G	Biovision		EUR 5677