Kash Biotech Express

Antibodies discovery, IHC

Recombinant Proteinase K, Molecular Biology Grade

cusao proteinse k

Capabilities

• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Elimination of DNases and RNases when isolating DNA and RNA from tissues or cell traces
• Dedication of enzyme localization
• Enhancing cloning effectivity of PCR merchandise

Proteinase Okay – one among many usually used enzymes in molecular biology.

Wonderful completely different for protein digestion in assorted options.

  • BLIRT – expert in Proteinase Okay manufacturing for over 15 years.
  • Licensed manufacturing course of ISO 13485 ensuing all through the best top of the range product with a really low batch-to-batch variability.
  • Enormous components, the possibility of white labeling, and portioning service – liquid selection all through the quantity on the shopper’s request (as lots as quite a lot of million samples month-to-month).

 

Thermo Scientific Proteinase Okay is a broad-range endolytic protease extensively used for digestion of proteins in nucleic acid preparations. It degrades proteins even all through the presence of detergents. Proteinase Okay cleaves peptide bonds on the carboxylic sides of aliphatic, fragrant, or hydrophobic amino acids. The Proteinase Okay is classed as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

Proteinase k kay (Lyophilized)

For Basic Digestion of Protein in Pure Samples

  • Lively over a pH differ of 4.3–12.0, in 0.5% SDS or 1% Triton® X-100
  • Purified to take away RNase and DNase actions

Secure at Room Temperature and Simple to Use

  • Lively over pH differ 4.3–12.Zero in 0.5% SDS or 1% Triton X-100
  • Retains >80% prepare at temperatures as lots as 60°C
  • No resuspension or thawing ahead of use
  • Cat.# MC5005, MC5008 provided at a highlight of 20mg/ml
Proteinase K, recombinant
Proteinase K, recombinant



Highlights

• Prepared-to-use reply
• Lively in a variety of response circumstances



Bear in mind

• The really useful working focus of Proteinase Okay is 0.05 to 1 mg/mL. The prepare of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to Four M urea
• Ca2+ protects Proteinase Okay in path of autolysis, will enhance the thermal stability, and has a regulatory perform for the substrate binding web site of Proteinase Okay
• Secure over a giant pH differ: 4.Zero to 12.5, optimum pH 7.5 to eight.0
• Optimum prepare at 50 to55°C
• Speedy denaturation of enzyme happens at temperatures above 65°C.

 

Proteinase Okay Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It’s a broad-spectrum endopeptidase with a really excessive particular prepare.

Recombinant Proteinase Okay enzyme is expressed in Pichia pastoris, and undergoes in depth purification to yield the simplest top of the range product.

Proteinase Okay Molecular Biology Grade (PCR Grade) is filled with life beneath a variety of response circumstances, together with elevated temperatures and the presence of SDS. In consequence, this enzyme is extensively utilizing for the digestion of proteins, together with DNases and RNases, all by way of nucleic acid preparations with out compromising the integrity of remoted DNA or RNA.

Selections

  • Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
  • Excessive prepare and distinctive purity.
  • Lively at excessive temperatures (as lots as 56 °C) and denaturing circumstances (e.g. all through the presence of urea and/or SDS), which makes it best for digesting proteins in quite a lot of options.
  • Secure over a giant pH differ: 4.0–12.5 (optimum pH 7.5–8.0).
  • Decreased quantity of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
  • Accessible as powder, lyophilized “cake” or liquid.

Capabilities:

  • Extraction of DNA and RNA from fully completely completely different beginning offers.
  • Purification of goal provides from contaminating proteins.
  • Elimination of DNases and RNases all by way of nucleic acids isolation.

 

Proteinase K
AR0056 5mL
EUR 121.2

Proteinase K
G029 1.0 ml
EUR 170.4

Proteinase K
GE0990-100MG 100 mg
EUR 112.8

Proteinase K
GE0990-1G 1 g
EUR 495.6

Proteinase K
GE0990-250MG 250 mg
EUR 184.8

Proteinase K
GE0990-25MG 25 mg
EUR 69.6

Proteinase K
GE0990-500MG 500 mg
EUR 298.8

Proteinase K
K1037-1 1 ml
EUR 80.4
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.

Proteinase K
K1037-5 5 ml
EUR 129.6
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.

Proteinase K
P2180-010 100mg
EUR 218.4

Proteinase K
P2180-050 500mg
EUR 448.8

Proteinase K
P2180-100 1g
EUR 627.6

Proteinase K
Z5050002 100 mg
EUR 205.2
Description: No genetic downstream application can do without the good old Proteinase K.

Proteinase K
M-CO-404 100 mg
EUR 376
Description: Proteinase K

PROTEINASE K SOLUTION
501-PK 5 ml
EUR 182.4
Description: Genomic PCR Grade Proteinase K Solution

Proteinase K protein
30R-2901 500 mg
EUR 510
Description: Purified recombinant Human Proteinase K protein

Proteinase K Solution
BIO-37084 5ml @ 20mg/ml Ask for price

Proteinase K Solution
BIO-37085 5 x 5ml @ 20mg/ml Ask for price

Proteinase K solution
GZ5904-100ML 100 ml
EUR 1088.4

Proteinase K solution
GZ5904-25ML 25 ml
EUR 418.8

Proteinase K - 1g
PB0451 1g
EUR 667.61

10x Proteinase K
TG4039 0.5ml
EUR 160.8

Proteinase K Antibody
6210-100 each
EUR 352.8

Proteinase K Antibody
6210-30T each
EUR 175.2

PROTEINASE K SOLUTION 10ML
502-PK 10 ml
EUR 286.8
Description: Genomic PCR Grade Proteinase K Solution

PROTEINASE K SOLUTION 25ML
503-PK 25 ml
EUR 602.4
Description: Genomic PCR Grade Proteinase K Solution

Proteinase K, 20mg/ml
P2170-002 2X1ml
EUR 181.2

Proteinase K, 20mg/ml
P2170-005 5X1ml
EUR 238.8

Proteinase K (high conc.)
RP02503 100mg Ask for price

Proteinase K 100 mg
DD91553 EACH
EUR 159.6

Proteinase K from Tritirachium album
abx082503-100mg 100 mg
EUR 276

Proteinase K, Recombinant, Molecular Grade
9210-100 each
EUR 144

Proteinase K, Recombinant, Molecular Grade
9210-10G each
EUR 3171.6

Proteinase K, Recombinant, Molecular Grade
9210-1G each
EUR 405.6

Proteinase K, Recombinant, Molecular Grade
9210-500 each
EUR 314.4

Proteinase K, Recombinant, Molecular Grade
9210-50G each Ask for price