Recombinant Proteinase K, Molecular Biology Grade

Capabilities
• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Elimination of DNases and RNases when isolating DNA and RNA from tissues or cell traces
• Dedication of enzyme localization
• Enhancing cloning effectivity of PCR merchandise
Proteinase Okay – one among many usually used enzymes in molecular biology.
Wonderful completely different for protein digestion in assorted options.
- BLIRT – expert in Proteinase Okay manufacturing for over 15 years.
- Licensed manufacturing course of ISO 13485 ensuing all through the best top of the range product with a really low batch-to-batch variability.
- Enormous components, the possibility of white labeling, and portioning service – liquid selection all through the quantity on the shopper’s request (as lots as quite a lot of million samples month-to-month).
Thermo Scientific Proteinase Okay is a broad-range endolytic protease extensively used for digestion of proteins in nucleic acid preparations. It degrades proteins even all through the presence of detergents. Proteinase Okay cleaves peptide bonds on the carboxylic sides of aliphatic, fragrant, or hydrophobic amino acids. The Proteinase Okay is classed as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.
Proteinase k kay (Lyophilized)
For Basic Digestion of Protein in Pure Samples
- Lively over a pH differ of 4.3–12.0, in 0.5% SDS or 1% Triton® X-100
- Purified to take away RNase and DNase actions
Secure at Room Temperature and Simple to Use
- Lively over pH differ 4.3–12.Zero in 0.5% SDS or 1% Triton X-100
- Retains >80% prepare at temperatures as lots as 60°C
- No resuspension or thawing ahead of use
- Cat.# MC5005, MC5008 provided at a highlight of 20mg/ml

Highlights
• Prepared-to-use reply
• Lively in a variety of response circumstances
Bear in mind
• The really useful working focus of Proteinase Okay is 0.05 to 1 mg/mL. The prepare of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to Four M urea
• Ca2+ protects Proteinase Okay in path of autolysis, will enhance the thermal stability, and has a regulatory perform for the substrate binding web site of Proteinase Okay
• Secure over a giant pH differ: 4.Zero to 12.5, optimum pH 7.5 to eight.0
• Optimum prepare at 50 to55°C
• Speedy denaturation of enzyme happens at temperatures above 65°C.
Proteinase Okay Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It’s a broad-spectrum endopeptidase with a really excessive particular prepare.
Recombinant Proteinase Okay enzyme is expressed in Pichia pastoris, and undergoes in depth purification to yield the simplest top of the range product.
Proteinase Okay Molecular Biology Grade (PCR Grade) is filled with life beneath a variety of response circumstances, together with elevated temperatures and the presence of SDS. In consequence, this enzyme is extensively utilizing for the digestion of proteins, together with DNases and RNases, all by way of nucleic acid preparations with out compromising the integrity of remoted DNA or RNA.
Selections
- Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
- Excessive prepare and distinctive purity.
- Lively at excessive temperatures (as lots as 56 °C) and denaturing circumstances (e.g. all through the presence of urea and/or SDS), which makes it best for digesting proteins in quite a lot of options.
- Secure over a giant pH differ: 4.0–12.5 (optimum pH 7.5–8.0).
- Decreased quantity of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
- Accessible as powder, lyophilized “cake” or liquid.
Capabilities:
- Extraction of DNA and RNA from fully completely completely different beginning offers.
- Purification of goal provides from contaminating proteins.
- Elimination of DNases and RNases all by way of nucleic acids isolation.
![]() Proteinase K |
|||
abx082041-100mg | Abbexa | 100 mg | EUR 258 |
![]() Proteinase K |
|||
GE0990-100MG | Glentham Life Sciences | 100 mg | EUR 94 |
![]() Proteinase K |
|||
GE0990-1G | Glentham Life Sciences | 1 g | EUR 413 |
![]() Proteinase K |
|||
GE0990-250MG | Glentham Life Sciences | 250 mg | EUR 154 |
![]() Proteinase K |
|||
GE0990-25MG | Glentham Life Sciences | 25 mg | EUR 58 |
![]() Proteinase K |
|||
GE0990-500MG | Glentham Life Sciences | 500 mg | EUR 249 |
![]() Proteinase K |
|||
P2180-010 | GenDepot | 100mg | EUR 182 |
![]() Proteinase K |
|||
P2180-050 | GenDepot | 500mg | EUR 374 |
![]() Proteinase K |
|||
P2180-100 | GenDepot | 1g | EUR 523 |
![]() Proteinase K |
|||
BIO-37037 | Bioline | 100mg | Ask for price |
![]() Proteinase K |
|||
BIO-37039 | Bioline | 1000mg | Ask for price |
![]() Proteinase K |
|||
K1037-1 | ApexBio | 1 ml | EUR 67 |
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease. |
![]() Proteinase K |
|||
K1037-5 | ApexBio | 5 ml | EUR 108 |
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease. |
![]() Proteinase K protein |
|||
30R-2901 | Fitzgerald | 500 mg | EUR 425 |
Description: Purified recombinant Human Proteinase K protein |
![]() Proteinase K Antibody |
|||
6210-100 | Biovision | EUR 294 |
![]() Proteinase K Antibody |
|||
6210-30T | Biovision | EUR 146 |
![]() PROTEINASE K SOLUTION |
|||
501-PK | Viagen | 5 ml | EUR 152 |
Description: Genomic PCR Grade Proteinase K Solution |
![]() Proteinase K solution |
|||
GZ5904-100ML | Glentham Life Sciences | 100 ml | EUR 907 |
![]() Proteinase K solution |
|||
GZ5904-25ML | Glentham Life Sciences | 25 ml | EUR 349 |
![]() Proteinase K Solution |
|||
BIO-37084 | Bioline | 5ml @ 20mg/ml | Ask for price |
![]() Proteinase K Solution |
|||
BIO-37085 | Bioline | 5 x 5ml @ 20mg/ml | Ask for price |
![]() Proteinase K - 1g |
|||
PB0451 | Bio Basic | 1g | EUR 556.34 |
![]() 10x Proteinase K |
|||
TG4039 | TopoGen | 0.5ml | EUR 134 |
![]() PROTEINASE K SOLUTION 10ML |
|||
502-PK | Viagen | 10 ml | EUR 239 |
Description: Genomic PCR Grade Proteinase K Solution |
![]() PROTEINASE K SOLUTION 25ML |
|||
503-PK | Viagen | 25 ml | EUR 502 |
Description: Genomic PCR Grade Proteinase K Solution |
![]() Proteinase K, 20mg/ml |
|||
P2170-002 | GenDepot | 2X1ml | EUR 151 |
![]() Proteinase K, 20mg/ml |
|||
P2170-005 | GenDepot | 5X1ml | EUR 199 |
![]() Proteinase K (high conc.) |
|||
RP02503 | Abclonal | 100mg | Ask for price |
![]() Proteinase K, Recombinant, Molecular Grade |
|||
9210-100 | Biovision | EUR 120 |
![]() Proteinase K, Recombinant, Molecular Grade |
|||
9210-10G | Biovision | EUR 2643 |
![]() Proteinase K, Recombinant, Molecular Grade |
|||
9210-1G | Biovision | EUR 338 |
![]() Proteinase K, Recombinant, Molecular Grade |
|||
9210-500 | Biovision | EUR 262 |
![]() Proteinase K, Recombinant, Molecular Grade |
|||
9210-50G | Biovision | Ask for price |
![]() Proteinase K, Recombinant, NGS Grade |
|||
9247-100 | Biovision | EUR 175 |
![]() Proteinase K, Recombinant, NGS Grade |
|||
9247-10G | Biovision | EUR 5677 |
![]() Proteinase K, Recombinant, NGS Grade |
|||
9247-1G | Biovision | EUR 675 |