Ultra Rainbow

SPHEROT -Ultra Rainbow Fluorescent Particles

New motion cytometers, with fluorescent channels from the UV to Far Pink, and corresponding fluorescent conjugates
in the mean time are accessible. In consequence, we now have developed the Extraordinarily Rainbow Fluorescent Particles with enhanced UV
and Far Pink fluorescence depth. The Extraordinarily Rainbow Fluorescent Particles embody a single peak and are
designed for checking the optical alignment of any motion cytometer in all channels.

Spherotech Cat. No. URFP-30-2 Instructions for use:
A. Preparation of Particles
1. Vortex the particles vigorously
2. Add 2 to 4 drops of particles to 1mL of sheath fluid or DI water. The inclusion of a small amount of detergent (~0.01%) throughout the dilution buffer will enhance the proportion of the singlet inhabitants.

B. Every day Alignment
To search out out the optical alignment of the system perform the subsequent:
1. Set a keep gate for the singlet inhabitants on the FSC vs SSC histogram to exclude aggregates
2. Modify the Purchase and Extreme voltage so that the indicate channel number of the peak is in a predetermined place on each histogram of curiosity. The histograms on the sooner net web page will be utilized as a info.
3. Accumulate 5000 events contained within the gate
4. Report the % CV and Extreme Voltage for all fluorescence channels of curiosity.
5. Use a computer program resembling Excel to generate the Levy Jennings graphs.

NOTE: If the values on any parameter exceed these of the day-to-day widespread or preset values, which are determined by not lower than one months worth of data, additional calibration or alignment procedures have to be carried out in response to the instrument operation handbook.

Ultra Rainbow
Extremely Rainbow

Motion Cytometry Alignment Particles

Spherotech offers all types of particles for determining if a motion cytometer is accurately aligned and has a transparent motion cell with no fluidic blockage. Using SPHERO Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions could also be measured to search out out the alignment and efficiency of the motion cytometer. Additiona data could also be found at STN-8: Calibration and Effectivity Monitoring of Motion Cytometer Using SPHERO Calibration Particles.

 

SPHERO™ Extraordinarily Rainbow Fluorescent Particles – A single inhabitants of Rainbow Fluorescent Particles with enhanced UV and Far Pink fluorescence depth
Catalog No.
Description
Focus
Nominal Dimension
Bundle Dimension
Value
URFP-02-2
Extraordinarily Rainbow Fluorescent Particles
1010/mL
0.1-0.Three µm
2 mL
$250.00
URFP-05-2
Extraordinarily Rainbow Fluorescent Particles
107/mL
0.4-0.6 µm
2 mL
$250.00
URFP-10-5
Extraordinarily Rainbow Fluorescent Particles
107/mL
1.0-1.4 µm
5 mL
$250.00
URFP-30-2
Extraordinarily Rainbow Fluorescent Particles
107/mL
3.0-3.4 µm
2 mL
$250.00
URFP-30-20
Extraordinarily Rainbow Fluorescent Particles
107/mL
3.0-3.4 µm
20 mL
$2000.00
URFP01-30-2K Extraordinarily Rainbow Fluorescent Particles 106/mL 3.0-3.4 µm 2×15 mL $355.00
URFP01-30-10Okay Extraordinarily Rainbow Fluorescent Particles 106/mL 3.0-3.4 µm 10×3 mL $410.00
URFP-38-2
Extraordinarily Rainbow Fluorescent Particles
107/mL
3.5-3.9 µm
2 mL
$255.00
URFP-38-10
Extraordinarily Rainbow Fluorescent Particles
107/mL
3.5-3.9 µm
10 mL
$1000.00
URFP-38-5A
Extraordinarily Rainbow Fluorescent Particles, Mid-Fluctuate
107/mL
3.5-3.9 µm
5 mL
$565.00
URFP-100-2
Extraordinarily Rainbow Fluorescent Particles
107/mL
8.1-12.Zero µm
2 mL
$285.00
URFP-150-2
Extraordinarily Rainbow Fluorescent Particles
5×106/mL
13.0-17.9 µm
2 mL
$285.00
URFP-200-5
Extraordinarily Rainbow Fluorescent Particles
1.0% w/v
18.0-24.9 µm
5 mL
$290.00
URFP-300-5
Extraordinarily Rainbow Fluorescent Particles
1.0% w/v
25.0-35.Zero µm
5 mL
$290.00

 

Description
Extraordinarily Rainbow Fluorescent Particles (by Spherotech™) are internally labeled with quite a few fluorophores. They exhibit full spectrum fluorescence, along with enhanced UV and Far Pink fluorescence. The mixture of fluorophores permits Extraordinarily Rainbow particles to be excited at any wavelength from 365 to 650nm. These particles will be present in two diameters, ~3.8µm and ~10.2µm.

Traits
Indicate Diameter: ~3.8µm (3.61-3.99µm) or ~10.2µm (8.1 – 12.0µm); see your Certificates of Analysis for exact measurement.
Focus: ~2 x 106particles/mL

Storage Buffer: Deionized water with 0.02% sodium azide and 0.01% NP-4O
Course of
A dilution of 1-2 drops to 1mL will current an sufficient number of particles for motion cytometry use.

 

Notes: Shake vigorously or vortex briefly sooner than use. Inclusion of a small amount of detergent throughout the diluent will help enhance the number of singlets. Diluted particles could also be saved throughout the fridge for future use.

KiloGreen 2X qPCR MasterMix-Low ROX

MasterMix-KL 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-Low ROX

MasterMix-LR 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen miRNA qPCR MasterMix-Low ROX

MasterMix-mL 4 x 1.25 ml for 500 reactions
EUR 151

TaqProbe 2X qPCR MasterMix-Low ROX

MasterMix-PL 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen Express 2X qPCR MasterMix-Low ROX

MasterMix-EL 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

Green-2-Go 1-Step qRT-PCR Mastermix-Low Rox

QPCR012-LR 100Rxn
EUR 239.23
  • Product category: PCR Related/PCR Premix (qRT-PCR)

BrightGreen 2X qPCR MasterMix-Low ROX

MasterMix-LR-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

One-Step BrightGreen qRT-PCR-Low ROX

G471-LR 100 rxn (20 ul/rxn)
EUR 158

One-Step TaqProbe qRT-PCR-Low ROX

G493-PL 100 rxn (20 ul/rxn)
EUR 158

TaqProbe 5X qPCR MasterMix-ROX

MasterMix-5P 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 5X qPCR MasterMix-ROX

MasterMix-5R 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

KiloGreen 2X qPCR MasterMix-ROX

MasterMix-KR 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

BrightGreen miRNA qPCR MasterMix-ROX

MasterMix-mR 4 x 1.25 ml for 500 reactions
EUR 151

TaqProbe 2X qPCR MasterMix-ROX

MasterMix-P 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-ROX

MasterMix-R 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

ExCellenCT One-Step BrightGreen qRT-PCR-Low ROX

G917-LR 25 Preps 100 x 20 ul reactions
EUR 177

ExCellenCT One-Step TaqProbe qRT-PCR-Low ROX

G918-PL 25 Preps 100 x 20 ul reactions
EUR 177

BrightGreen Express 2X qPCR MasterMix-ROX

MasterMix-ER 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

Jade? QRT Kit-Low ROX

M1177-100
EUR 370

Taqman QRT Kit-Low ROX

M1185-100
EUR 370

BrightGreen 2X qPCR MasterMix-ROX

MasterMix-R-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

Green-2-Go 1-Step qRT-PCR Mastermix-Rox (100X20ul Rxn)

QPCR010-R 100Rxn
EUR 239.23
  • Product category: PCR Related/PCR Premix (qRT-PCR)

Leave a Reply

Your email address will not be published. Required fields are marked *