Artificial Antibody Mimics Primarily based on Most cancers-Concentrating on Immunostimulatory Peptides
De novo cancer-targeting immunostimulatory peptides have been designed and developed as synthetic antibody mimics. A selected trimeric peptide displayed binding on GRP78 + HepG2 and A549 aim cells with some extent of GRP78-binding dependence.
Equally, the chosen trimeric peptide was moreover found to exhibit NK cell binding in an NKp30-dependent technique which translated into NK cell activation as indicated by cytokine secretion. In co-culture, fluorescence microscopy revealed that the aim GFP-expressing A549 cells have been visibly associated to the effector NK cells when pre-activated with lead trimeric peptide.
Accordingly, A549 cells have been found to be compromised as evidenced by the dearth of GFP signal and notable detection of early/late stage apoptosis. Investigation of immunological markers related to toxicity revealed detectable secretion of pro-inflammatory cytokines and chemokines, along with IFN-γ, TNF-α, and IL-8.
Furthermore, administration of peptide-activated NK cells into A549-tumor bearing mice resulted in a relentless decrease in tumor improvement when compared with the untreated administration group. Taken collectively, the identification of a lead trimeric peptide in a position to specializing in and activating NK cells’ immunotoxicity straight within the path of GRP78 + /B7H6 – tumors provides a novel proof-of-concept for the occasion of cancer-targeting immunostimulatory peptide ligands that mimic antibody specializing in and activating options related to most cancers immunotherapy functions.
Variable anti-HBs antibodytiters in vaccinated supply cohorts – A cross-sectional population-based analysis
Background: After the introduction of hepatitis B (HB) vaccination in 1995 in newborns, two catch-up campaigns targeted unvaccinated 9 12 months earlier in 2000-2003 (born 1991-1994) and the 18 12 months earlier in 2004-2008 (born 1986-1990), resulting in various birth-cohorts. Our aim was to guage the anti-HBs titers in each birth-cohort.
Methods: We included all outpatients (78.5%) and hospitalized victims with measured anti-HBs antibody titers throughout the Educating Hospital of Infectious Illnesses, Cluj-Napoca, Romania, all through April 2014 – December 2018 (with out HB historic previous). We in distinction the anti-HBs titers in all birth-cohorts using the Lexis surfaces (titers by age, time interval and cohort patterns). We moreover evaluated the number of acute HB throughout the corresponding inpatient birth-cohorts and specific groups.
Outcomes: We included 2963 contributors, suggest age = 31.0 ± 14.2, 64.1% ladies. The birth-cohort 1995-2006, vaccinated after provide (n = 424, 3-dose HB vaccine safety > 90%), had significantly lower defending titers (41.3% >10 mIU/mL) compared with the alternative birth-cohorts: born after 2007 (moreover vaccinated at supply, 67.0%, n = 106), 1991-1994 (age 9, 74.3%, n = 847), 1986-1990 (age 18, 71.3%, n = 543). Inside the unvaccinated cohort (n = 1043, suggest age = 45.5 ± 12.4) defending titers have been current in 44.8%, most probably after self-limited HB an an infection.
Concordant outcomes have been found using the proportion of victims with detectable or sturdy titers, and median or geometric suggest titers. Four breakthrough acute HB infections have been hospitalized of the corresponding vaccinated cohorts (supply years 1988, 1990, 1995, 1996). Data on only a few examined infants (n = 47, not included within the precept analysis) demonstrated good security, 88.9%.
Conclusions: Our analysis demonstrated the prolonged-term proof of security of HBV vaccine at 20 years following the primary immunization and a booster seems unsupported. Extra analysis should be carried out to guage the need of a booster dose inside the traditional inhabitants and specific groups.
Anti-complement difficulty I antibody associated atypical hemolytic uremic syndrome – A model new notion for future perspective!
- Atypical hemolytic uremic syndrome (aHUS) is induced primarily by complement dysregulation. Although quite a few defects throughout the complement system explaining pathophysiology have been described in latest occasions, the etiology nonetheless stays unclear in about thirty % of situations.
- In exploring completely different causes, very similar to anti- complement difficulty H (anti-CFH) antibody associated HUS, we hypothesized that anti-complement difficulty I (anti-CFI) antibody could play a activity in aHUS.
- Extra, we tried to clarify the scientific profile and consequence of those with extreme anti CFI antibody titers. Eleven of thirty 5 youngsters (31 %) acknowledged with aHUS from July 2017 to December 2018 had extreme IgG anti-CFI antibody titers. Median age was 10 months (6, 33) with no intercourse distinction. Thirty-six % (4/11) had nephrotic-range proteinuria. C3 was low in Eight youngsters (72.7 %) with suggest C3 (68.1 ± 14.7 mg/dL).
- Plasmapheresis was carried out in 2 youngsters who promptly responded, suggesting the potential operate of anti-CFI antibody in pathogenesis of aHUS in these victims. Extra analysis analyzing operate of anti-CFI antibodies in aHUS is warranted with longitudinal and genetic analysis.
 ELISA Kit) Mouse Clusterin (CLU) ELISA Kit |
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| RD-CLU-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 441.6 |
Mouse Clusterin (CLU) ELISA Kit |
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| RD-CLU-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 604.8 |
Mouse Clusterin (CLU) ELISA Kit |
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| RDR-CLU-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 460.8 |
Mouse Clusterin (CLU) ELISA Kit |
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| RDR-CLU-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 632.4 |
 ELISA Kit) Canine Clusterin (CLU) ELISA Kit |
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| DLR-CLU-c-48T | DL Develop | 48T | EUR 658.8 |
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Description: A sandwich quantitative ELISA assay kit for detection of Canine Clusterin (CLU) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Canine Clusterin (CLU) ELISA Kit |
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| DLR-CLU-c-96T | DL Develop | 96T | EUR 861.6 |
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Description: A sandwich quantitative ELISA assay kit for detection of Canine Clusterin (CLU) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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 ELISA Kit) Human Clusterin (CLU) ELISA Kit |
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| DLR-CLU-Hu-48T | DL Develop | 48T | EUR 470.4 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Clusterin (CLU) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Human Clusterin (CLU) ELISA Kit |
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| DLR-CLU-Hu-96T | DL Develop | 96T | EUR 602.4 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Clusterin (CLU) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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 ELISA Kit) Rat Clusterin (CLU) ELISA Kit |
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| DLR-CLU-Ra-48T | DL Develop | 48T | EUR 633.6 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Clusterin (CLU) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Rat Clusterin (CLU) ELISA Kit |
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| DLR-CLU-Ra-96T | DL Develop | 96T | EUR 828 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Clusterin (CLU) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Canine Clusterin (CLU) ELISA Kit |
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| RD-CLU-c-48Tests | Reddot Biotech | 48 Tests | EUR 668.4 |
Canine Clusterin (CLU) ELISA Kit |
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| RD-CLU-c-96Tests | Reddot Biotech | 96 Tests | EUR 930 |
Human Clusterin (CLU) ELISA Kit |
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| RD-CLU-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 456 |
Human Clusterin (CLU) ELISA Kit |
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| RD-CLU-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 625.2 |
Rat Clusterin (CLU) ELISA Kit |
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| RD-CLU-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 640.8 |
Rat Clusterin (CLU) ELISA Kit |
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| RD-CLU-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 890.4 |
Canine Clusterin (CLU) ELISA Kit |
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| RDR-CLU-c-48Tests | Reddot Biotech | 48 Tests | EUR 699.6 |
Canine Clusterin (CLU) ELISA Kit |
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| RDR-CLU-c-96Tests | Reddot Biotech | 96 Tests | EUR 973.2 |
Human Clusterin (CLU) ELISA Kit |
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| RDR-CLU-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 475.2 |
Human Clusterin (CLU) ELISA Kit |
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| RDR-CLU-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 654 |
Rat Clusterin (CLU) ELISA Kit |
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| RDR-CLU-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 669.6 |
Rat Clusterin (CLU) ELISA Kit |
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| RDR-CLU-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 931.2 |
 Monoclonal Antibody (Pig)) Clusterin (CLU) Monoclonal Antibody (Pig) |
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| 4-MAB180Po21 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Pig Clusterin (CLU) |
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 Monoclonal Antibody (Rat)) Clusterin (CLU) Monoclonal Antibody (Rat) |
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| 4-MAB180Ra21 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rat Clusterin (CLU) |
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 Monoclonal Antibody (Rabbit)) Clusterin (CLU) Monoclonal Antibody (Rabbit) |
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| 4-MAB180Rb21 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rabbit Clusterin (CLU) |
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 Antibody) Clusterin (CLU) Antibody |
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| 20-abx171801 | Abbexa |
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Clusterin (CLU) Antibody |
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| 20-abx137211 | Abbexa |
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Clusterin (CLU) Antibody |
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| abx139360-01mg | Abbexa | 0.1 mg | EUR 477.6 |
Clusterin (CLU) Antibody |
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| 20-abx100235 | Abbexa |
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Clusterin (CLU) Antibody |
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| 20-abx100236 | Abbexa |
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Clusterin (CLU) Antibody |
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| 20-abx100237 | Abbexa |
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Clusterin (CLU) Antibody |
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| 20-abx100238 | Abbexa |
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Clusterin (CLU) Antibody |
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| 20-abx100239 | Abbexa |
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Clusterin (CLU) Antibody |
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| 20-abx100240 | Abbexa |
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Clusterin (CLU) Antibody |
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| 20-abx100241 | Abbexa |
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Clusterin (CLU) Antibody |
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| 20-abx100242 | Abbexa |
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Clusterin (CLU) Antibody |
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| 20-abx100243 | Abbexa |
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Clusterin (CLU) Antibody |
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| 20-abx104829 | Abbexa |
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Clusterin (CLU) Antibody |
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| 20-abx001253 | Abbexa |
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Clusterin (CLU) Antibody |
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| 20-abx109653 | Abbexa |
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Clusterin (CLU) Antibody |
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| 20-abx130424 | Abbexa |
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Clusterin (CLU) Antibody |
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| 20-abx111697 | Abbexa |
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Clusterin (CLU) Antibody |
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| abx018139-100ug | Abbexa | 100 ug | EUR 460.8 |
Clusterin (CLU) Antibody |
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| abx026000-400ul | Abbexa | 400 ul | EUR 627.6 |
Clusterin (CLU) Antibody |
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| abx026000-80l | Abbexa | 80 µl | EUR 343.2 |
Clusterin (CLU) Antibody |
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| abx036348-100ug | Abbexa | 100 ug | EUR 469.2 |
Clusterin (CLU) Antibody |
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| abx036349-100ug | Abbexa | 100 ug | EUR 469.2 |
Clusterin (CLU) Antibody |
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| 20-abx329365 | Abbexa |
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Clusterin (CLU) Antibody |
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| 20-abx225116 | Abbexa |
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Clusterin (CLU) Antibody |
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| abx430866-200ul | Abbexa | 200 ul | EUR 460.8 |
Clusterin (CLU) Antibody |
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| abx430867-200ul | Abbexa | 200 ul | EUR 460.8 |
Clusterin (CLU) Antibody |
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| abx430877-200ul | Abbexa | 200 ul | EUR 460.8 |
Clusterin (CLU) Antibody |
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| abx430878-200ul | Abbexa | 200 ul | EUR 460.8 |
Clusterin (CLU) Antibody |
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| abx231781-100ug | Abbexa | 100 ug | EUR 577.2 |
Clusterin (CLU) Antibody |
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| abx231782-100ug | Abbexa | 100 ug | EUR 577.2 |
Clusterin (CLU) Antibody |
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| 20-abx210640 | Abbexa |
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Clusterin (CLU) Antibody |
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| 20-abx175911 | Abbexa |
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 Monoclonal Antibody (Human, Pig)) Clusterin (CLU) Monoclonal Antibody (Human, Pig) |
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| 4-MAB180Hu22 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human, Pig Clusterin (CLU) |
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 Monoclonal Antibody (Pig), APC) Clusterin (CLU) Monoclonal Antibody (Pig), APC |
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| 4-MAB180Po21-APC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Pig Clusterin (CLU). This antibody is labeled with APC. |
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 Monoclonal Antibody (Pig), Biotinylated) Clusterin (CLU) Monoclonal Antibody (Pig), Biotinylated |
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| 4-MAB180Po21-Biotin | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Pig Clusterin (CLU). This antibody is labeled with Biotin. |
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 Monoclonal Antibody (Pig), Cy3) Clusterin (CLU) Monoclonal Antibody (Pig), Cy3 |
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| 4-MAB180Po21-Cy3 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Pig Clusterin (CLU). This antibody is labeled with Cy3. |
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 Monoclonal Antibody (Pig), FITC) Clusterin (CLU) Monoclonal Antibody (Pig), FITC |
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| 4-MAB180Po21-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Pig Clusterin (CLU). This antibody is labeled with FITC. |
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 Monoclonal Antibody (Pig), HRP) Clusterin (CLU) Monoclonal Antibody (Pig), HRP |
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| 4-MAB180Po21-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Pig Clusterin (CLU). This antibody is labeled with HRP. |
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 Monoclonal Antibody (Pig), PE) Clusterin (CLU) Monoclonal Antibody (Pig), PE |
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| 4-MAB180Po21-PE | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Pig Clusterin (CLU). This antibody is labeled with PE. |
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 Monoclonal Antibody (Rat), APC) Clusterin (CLU) Monoclonal Antibody (Rat), APC |
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| 4-MAB180Ra21-APC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rat Clusterin (CLU). This antibody is labeled with APC. |
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 Monoclonal Antibody (Rat), Biotinylated) Clusterin (CLU) Monoclonal Antibody (Rat), Biotinylated |
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| 4-MAB180Ra21-Biotin | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rat Clusterin (CLU). This antibody is labeled with Biotin. |
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 Monoclonal Antibody (Rat), Cy3) Clusterin (CLU) Monoclonal Antibody (Rat), Cy3 |
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| 4-MAB180Ra21-Cy3 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rat Clusterin (CLU). This antibody is labeled with Cy3. |
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 Monoclonal Antibody (Rat), FITC) Clusterin (CLU) Monoclonal Antibody (Rat), FITC |
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| 4-MAB180Ra21-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rat Clusterin (CLU). This antibody is labeled with FITC. |
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 Monoclonal Antibody (Rat), HRP) Clusterin (CLU) Monoclonal Antibody (Rat), HRP |
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| 4-MAB180Ra21-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rat Clusterin (CLU). This antibody is labeled with HRP. |
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 Monoclonal Antibody (Rat), PE) Clusterin (CLU) Monoclonal Antibody (Rat), PE |
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| 4-MAB180Ra21-PE | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rat Clusterin (CLU). This antibody is labeled with PE. |
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 Monoclonal Antibody (Rabbit), APC) Clusterin (CLU) Monoclonal Antibody (Rabbit), APC |
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| 4-MAB180Rb21-APC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rabbit Clusterin (CLU). This antibody is labeled with APC. |
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 Monoclonal Antibody (Rabbit), Biotinylated) Clusterin (CLU) Monoclonal Antibody (Rabbit), Biotinylated |
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| 4-MAB180Rb21-Biotin | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rabbit Clusterin (CLU). This antibody is labeled with Biotin. |
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 Monoclonal Antibody (Rabbit), Cy3) Clusterin (CLU) Monoclonal Antibody (Rabbit), Cy3 |
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| 4-MAB180Rb21-Cy3 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rabbit Clusterin (CLU). This antibody is labeled with Cy3. |
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 Monoclonal Antibody (Rabbit), FITC) Clusterin (CLU) Monoclonal Antibody (Rabbit), FITC |
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| 4-MAB180Rb21-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rabbit Clusterin (CLU). This antibody is labeled with FITC. |
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 Monoclonal Antibody (Rabbit), HRP) Clusterin (CLU) Monoclonal Antibody (Rabbit), HRP |
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| 4-MAB180Rb21-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rabbit Clusterin (CLU). This antibody is labeled with HRP. |
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