Development of Monoclonal Antibodies and Antigen-Capture ELISA for Human Parechovirus Type 3
- Human parechovirus kind 3 (HPeV3) is an etiologic agent of respiratory illnesses, meningitis, and sepsis-like sickness in each infants and adults.
- Monoclonal antibodies (mAbs) is usually a promising diagnostic software for antigenic illnesses corresponding to virus an infection, as they provide a excessive specificity towards a particular viral antigen. Nevertheless, so far, there isn’t any particular mAb accessible for the analysis of HPeV3 an infection.
- On this research, we developed and characterised mAbs particular for HPeV3 capsid protein VP0. We used cell-free, wheat germ-synthesized viral VP0 protein for immunizing BALB/c mice to generate hybridomas. From the resultant hybridoma clones, we chosen 9 clones producing mAbs reactive to the HPeV3-VP0 antigen, primarily based on enzyme-linked immunosorbent assay (ELISA).
- Epitope mapping confirmed that these mAbs acknowledged three distinct domains in HPeV3 VP0. Six mAbs acknowledged HPeV3 particularly and the opposite three mAbs confirmed cross-reactivity with different HPeVs.
- Utilizing the HPeV3-specific mAbs, we then developed an ELISA for viral antigen detection that may very well be reliably used for laboratory analysis of HPeV3. This ELISA system exhibited no cross-reactivity with different associated viruses. Our newly developed mAbs would, thus, present a helpful set of instruments for future analysis and guarantee HPeV3-specific analysis.
Growth and utility of a colloidal carbon check strip for the detection of antibodies in opposition to Mycoplasma bovis
Mycoplasma bovis (M. bovis) is a crucial bovine mycoplasma implicated in economically essential scientific illnesses, corresponding to respiratory illnesses, otitis media, and mastitis. The prevalence of M. bovis-associated mastitis in each cattle and buffaloes has been more and more acknowledged as a worldwide drawback. Excessive morbidity charges and consequential financial losses have been devastating to the affected cattle and buffalo farms, particularly these in growing international locations.
Subsequently, a speedy and correct methodology is urgently wanted to detect M. bovis. On this research, a speedy and easy lateral circulate strip for detecting antibodies in opposition to M. bovis was established that used carbon nanoparticles (CNPs) because the labelled supplies. The outcomes from the check strip have been extremely in line with these from ELISA.
The check confirmed excessive specificity (100%) and no cross-reaction with different bovine pathogens. The detection sensitivity of the check was additionally comparatively excessive (97.67%). All the outcomes indicated that the colloidal carbon check strip may function a easy, speedy, delicate, and particular diagnostic methodology for detecting antibodies in opposition to M. bovis at cattle farms.
Comparability of the analytical and scientific performances of 4 anti-cyclic citrullinated peptide antibody assays for diagnosing rheumatoid arthritis
Introduction/goals: Anti-cyclic citrullinated peptide antibody (anti-CCP) is likely one of the most essential serologic markers for diagnosing rheumatoid arthritis (RA). This research aimed to match the analytical and scientific performances of the second- and third-generation anti-CCP assays.
Strategies: 4 automated anti-CCP assays have been evaluated: Refrain anti-CCP (DiesseDiagnostica), Elecsys anti-CCP (Roche Diagnostics), Atellica IM anti-CCP IgG (Siemens Healthineers), and Quanta Flash® CCP3 (Inova Diagnostics Inc.). Analytical efficiency included the precision, linearity, correlation, and concordance fee. For evaluating the scientific efficiency, 240 affected person samples (120 constructive and 120 adverse samples, decided by the Refrain anti-CCP assay) have been used, together with these with a analysis of RA (n = 132) and non-RA (n = 108). Utilizing receiver working attribute (ROC) curve evaluation, the sensitivity and specificity have been evaluated.
Outcomes: All 4 assays that have been evaluated confirmed good precision and linearity, and their correlation and concordance charges have been in acceptable ranges. The world beneath the curve (AUC) values ranged from 0.888 to 0.914, exhibiting a superb diagnostic efficiency. The sensitivity and specificity of all assays have been comparable (88.0-97.2%).
Conclusions: All 4 anti-CCP assays confirmed good analytical and diagnostic performances for diagnosing RA. After adjusting the cutoff values, these assays are anticipated to point out enhanced sensitivity and specificity.
Key Factors • Earlier research have described the diagnostic efficiency of some immunologic markers in RA analysis, however nothing has been confirmed to be sufficiently good in scientific apply. • All 4 automated anti-CCP assays confirmed good analytical and diagnostic performances for diagnosing RA in scientific apply. • After adjusting the cutoff values, these assays are anticipated to point out enhanced sensitivity and specificity. • The current research supplies reassuring proof that any of the studied commercially accessible anti-CCP checks for detecting rheumatoid arthritis present comparable diagnostic data to establishments that undertake these particular testing methods.
Priming of pancreatic cancer cells with bispecific antibody armed activated T cells sensitizes tumors for enhanced chemoresponsiveness
In this study, we investigated the ability of bispecific antibody armed activated T cells to target drug resistant pancreatic cancer cells and whether or not “priming” these resistant cancer cells with bispecific antibody armed activated T cells could enhance subsequent responsiveness to chemotherapeutic drugs. Chemotherapeutic responses for pancreatic cancer are either limited or the tumors develop resistance to chemotherapy regimens. The impetus for this study was the remarkable clinical response seen in our earlier phase I/II clinical trial: a pancreatic cancer patient with drug resistant tumors who showed progression of disease following three infusions of anti-CD3 x anti-EGFR bispecific antibody armed activated T cells (EGFR BATs) was restarted on the initial low dose of <i>5-fluorouracil</i> showed complete response, suggesting that BATs infusions may have sensitized patient’s tumor for chemoresponsiveness.
In the current study, we tested the hypothesis that BATs can sensitize tumors for chemoresponsiveness. Gemcitabine or cisplatin-resistant MiaPaCa-2 and L3.6 cell lines were effectively targeted by EGFR BATs. Priming of drug sensitive or resistant cells with EGFR BATs followed by retargeting with lower concentrations of 50% inhibitory concentration of gemcitabine or cisplatin showed enhanced cytotoxicity. Gemcitabine or cisplatin-resistant cell lines show an increased proportion of CD44<sup>+</sup>/CD24<sup>+</sup>/EpCAM<sup>+</sup> cancer stem like cells as well as an increased number of ABC transporter ABCG2 positive cells compared to the parental cell lines. These data suggest that bispecific antibody armed activated T cells can target and kill chemo-resistant tumor cells and also markedly augment subsequent chemotherapeutic responsiveness, possibly by modulating the expression of ABC transporters.
Prediction and mitigation of mutation threats to COVID-19 vaccines and antibody therapies
Antibody therapeutics and vaccines are among our last resort to end the raging COVID-19 pandemic. They, however, are prone to over 5000 mutations on the spike (S) protein uncovered by a Mutation Tracker based on over 200 000 genome isolates. It is imperative to understand how mutations will impact vaccines and antibodies in development. In this work, we first study the mechanism, frequency, and ratio of mutations on the S protein which is the common target of most COVID-19 vaccines and antibody therapies. Additionally, we build a library of 56 antibody structures and analyze their 2D and 3D characteristics. Moreover, we predict the mutation-induced binding free energy (BFE) changes for the complexes of S protein and antibodies or ACE2. By integrating genetics, biophysics, deep learning, and algebraic topology, we reveal that most of the 462 mutations on the receptor-binding domain (RBD) will weaken the binding of S protein and antibodies and disrupt the efficacy and reliability of antibody therapies and vaccines.
A list of 31 antibody disrupting mutants is identified, while many other disruptive mutations are detailed as well. We also unveil that about 65% of the existing RBD mutations, including those variants recently found in the United Kingdom (UK) and South Africa, will strengthen the binding between the S protein and human angiotensin-converting enzyme 2 (ACE2), resulting in more infectious COVID-19 variants. We discover the disparity between the extreme values of RBD mutation-induced BFE strengthening and weakening of the bindings with antibodies and angiotensin-converting enzyme 2 (ACE2), suggesting that SARS-CoV-2 is at an advanced stage of evolution for human infection, while the human immune system is able to produce optimized antibodies. This discovery, unfortunately, implies the vulnerability of current vaccines and antibody drugs to new mutations. Our predictions were validated by comparison with more than 1400 deep mutations on the S protein RBD. Our results show the urgent need to develop new mutation-resistant vaccines and antibodies and to prepare for seasonal vaccinations.
 ELISA Kit) Equine Interleukin 8 (IL8) ELISA Kit |
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| DLR-IL8-Eq-48T | DL Develop | 48T | EUR 603.6 |
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Description: A sandwich quantitative ELISA assay kit for detection of Equine Interleukin 8 (IL8) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Equine Interleukin 8 (IL8) ELISA Kit |
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| DLR-IL8-Eq-96T | DL Develop | 96T | EUR 784.8 |
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Description: A sandwich quantitative ELISA assay kit for detection of Equine Interleukin 8 (IL8) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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 ELISA Kit) Fish Interleukin 8 (IL8) ELISA Kit |
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| DLR-IL8-Fi-48T | DL Develop | 48T | EUR 603.6 |
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Description: A sandwich quantitative ELISA assay kit for detection of Fish Interleukin 8 (IL8) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Fish Interleukin 8 (IL8) ELISA Kit |
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| DLR-IL8-Fi-96T | DL Develop | 96T | EUR 784.8 |
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Description: A sandwich quantitative ELISA assay kit for detection of Fish Interleukin 8 (IL8) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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 ELISA Kit) Human Interleukin 8 (IL8) ELISA Kit |
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| DLR-IL8-Hu-48T | DL Develop | 48T | EUR 399.6 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 8 (IL8) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Human Interleukin 8 (IL8) ELISA Kit |
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| DLR-IL8-Hu-96T | DL Develop | 96T | EUR 504 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 8 (IL8) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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 ELISA Kit) Porcine Interleukin 8 (IL8) ELISA Kit |
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| DLR-IL8-p-48T | DL Develop | 48T | EUR 537.6 |
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Description: A sandwich quantitative ELISA assay kit for detection of Porcine Interleukin 8 (IL8) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Porcine Interleukin 8 (IL8) ELISA Kit |
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| DLR-IL8-p-96T | DL Develop | 96T | EUR 694.8 |
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Description: A sandwich quantitative ELISA assay kit for detection of Porcine Interleukin 8 (IL8) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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 ELISA Kit) Rabbit Interleukin 8 (IL8) ELISA Kit |
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| DLR-IL8-Rb-48T | DL Develop | 48T | EUR 544.8 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Interleukin 8 (IL8) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Rabbit Interleukin 8 (IL8) ELISA Kit |
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| DLR-IL8-Rb-96T | DL Develop | 96T | EUR 704.4 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Interleukin 8 (IL8) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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 ELISA Kit) Monkey Interleukin 8 (IL8) ELISA Kit |
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| DLR-IL8-Si-48T | DL Develop | 48T | EUR 568.8 |
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Description: A sandwich quantitative ELISA assay kit for detection of Monkey Interleukin 8 (IL8) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Monkey Interleukin 8 (IL8) ELISA Kit |
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| DLR-IL8-Si-96T | DL Develop | 96T | EUR 736.8 |
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Description: A sandwich quantitative ELISA assay kit for detection of Monkey Interleukin 8 (IL8) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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 ELISA Kit) Bovine Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-b-48Tests | Reddot Biotech | 48 Tests | EUR 490.8 |
Bovine Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-b-96Tests | Reddot Biotech | 96 Tests | EUR 675.6 |
 ELISA Kit) Canine Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-c-48Tests | Reddot Biotech | 48 Tests | EUR 381.6 |
Canine Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-c-96Tests | Reddot Biotech | 96 Tests | EUR 519.6 |
 ELISA Kit) Chicken Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-Ch-48Tests | Reddot Biotech | 48 Tests | EUR 506.4 |
Chicken Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-Ch-96Tests | Reddot Biotech | 96 Tests | EUR 697.2 |
Equine Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-Eq-48Tests | Reddot Biotech | 48 Tests | EUR 633.6 |
Equine Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-Eq-96Tests | Reddot Biotech | 96 Tests | EUR 879.6 |
Fish Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-Fi-48Tests | Reddot Biotech | 48 Tests | EUR 633.6 |
Fish Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-Fi-96Tests | Reddot Biotech | 96 Tests | EUR 879.6 |
Human Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 391.2 |
Human Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 534 |
Porcine Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-p-48Tests | Reddot Biotech | 48 Tests | EUR 555.6 |
Porcine Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-p-96Tests | Reddot Biotech | 96 Tests | EUR 768 |
Rabbit Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-Rb-48Tests | Reddot Biotech | 48 Tests | EUR 564 |
Rabbit Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-Rb-96Tests | Reddot Biotech | 96 Tests | EUR 781.2 |
Monkey Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-Si-48Tests | Reddot Biotech | 48 Tests | EUR 591.6 |
Monkey Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-Si-96Tests | Reddot Biotech | 96 Tests | EUR 819.6 |
Bovine Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-b-48Tests | Reddot Biotech | 48 Tests | EUR 470.4 |
Bovine Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-b-96Tests | Reddot Biotech | 96 Tests | EUR 646.8 |
Canine Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-c-48Tests | Reddot Biotech | 48 Tests | EUR 366 |
Canine Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-c-96Tests | Reddot Biotech | 96 Tests | EUR 498 |
Chicken Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-Ch-48Tests | Reddot Biotech | 48 Tests | EUR 484.8 |
Chicken Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-Ch-96Tests | Reddot Biotech | 96 Tests | EUR 667.2 |
Equine Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-Eq-48Tests | Reddot Biotech | 48 Tests | EUR 606 |
Equine Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-Eq-96Tests | Reddot Biotech | 96 Tests | EUR 840 |
Human Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 375.6 |
Human Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 511.2 |
Porcine Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-p-48Tests | Reddot Biotech | 48 Tests | EUR 531.6 |
Porcine Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-p-96Tests | Reddot Biotech | 96 Tests | EUR 734.4 |
Rabbit Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-Rb-48Tests | Reddot Biotech | 48 Tests | EUR 540 |
Rabbit Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-Rb-96Tests | Reddot Biotech | 96 Tests | EUR 746.4 |
Monkey Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-Si-48Tests | Reddot Biotech | 48 Tests | EUR 566.4 |
Monkey Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-Si-96Tests | Reddot Biotech | 96 Tests | EUR 783.6 |
 Monoclonal Antibody (Horse)) Interleukin 8 (IL8) Monoclonal Antibody (Horse) |
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| 4-MAA080Eq21 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Horse Interleukin 8 (IL8) |
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 Monoclonal Antibody (Chicken)) Interleukin 8 (IL8) Monoclonal Antibody (Chicken) |
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| 4-MAA080Ga22 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Chicken Interleukin 8 (IL8) |
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 Monoclonal Antibody (Human)) Interleukin 8 (IL8) Monoclonal Antibody (Human) |
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| 4-MAA080Hu22 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Interleukin 8 (IL8) |
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Interleukin 8 (IL8) Monoclonal Antibody (Human) |
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| 4-MAA080Hu24 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Interleukin 8 (IL8) |
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 Monoclonal Antibody (Pig)) Interleukin 8 (IL8) Monoclonal Antibody (Pig) |
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| 4-MAA080Po22 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Pig Interleukin 8 (IL8) |
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Interleukin 8 (IL8) Monoclonal Antibody (Pig) |
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| 4-MAA080Po24 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Pig Interleukin 8 (IL8) |
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Interleukin 8 (IL8) Monoclonal Antibody (Pig) |
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| 4-MAA080Po26 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Pig Interleukin 8 (IL8) |
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 Monoclonal Antibody (Rabbit)) Interleukin 8 (IL8) Monoclonal Antibody (Rabbit) |
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| 4-MAA080Rb22 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rabbit Interleukin 8 (IL8) |
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 ELISA Kit) Guinea pig Interleukin 8 (IL8) ELISA Kit |
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| DLR-IL8-Gu-48T | DL Develop | 48T | EUR 465.6 |
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Description: A sandwich quantitative ELISA assay kit for detection of Guinea pig Interleukin 8 (IL8) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Guinea pig Interleukin 8 (IL8) ELISA Kit |
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| DLR-IL8-Gu-96T | DL Develop | 96T | EUR 595.2 |
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Description: A sandwich quantitative ELISA assay kit for detection of Guinea pig Interleukin 8 (IL8) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Guinea pig Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-Gu-48Tests | Reddot Biotech | 48 Tests | EUR 469.2 |
Guinea pig Interleukin 8 (IL8) ELISA Kit |
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| RDR-IL8-Gu-96Tests | Reddot Biotech | 96 Tests | EUR 645.6 |
Guinea pig Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-Gu-48Tests | Reddot Biotech | 48 Tests | EUR 450 |
Guinea pig Interleukin 8 (IL8) ELISA Kit |
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| RD-IL8-Gu-96Tests | Reddot Biotech | 96 Tests | EUR 618 |
 Antibody) Interleukin 8 (IL8) Antibody |
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| 20-abx137023 | Abbexa |
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Interleukin 8 (IL8) Antibody |
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| 20-abx100960 | Abbexa |
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Interleukin 8 (IL8) Antibody |
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| 20-abx100961 | Abbexa |
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Interleukin 8 (IL8) Antibody |
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| 20-abx100962 | Abbexa |
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Interleukin 8 (IL8) Antibody |
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| 20-abx100963 | Abbexa |
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