kashbiotech

NOTCH3-targeted antibody drug conjugates regress tumors by inducing apoptosis in receptor cells and through transendocytosis into ligand cells

 Aberrant NOTCH3 signaling and overexpression is oncogenic, related with most cancers stem cells and drug resistance, but therapeutic concentrating on stays elusive. Right here, we develop NOTCH3-targeted antibody drug conjugates (NOTCH3-ADCs) by bioconjugation of an auristatin microtubule inhibitor via a protease cleavable linker to 2 antibodies with differential skills to inhibit signaling. The signaling inhibitory antibody quickly induces ligand-independent receptor clustering and internalization via each caveolin and clathrin-mediated pathways.

The non-inhibitory antibody additionally effectively endocytoses through clathrin with out inducing receptor clustering however with slower lysosomal co-localization kinetics. As well as, DLL4 ligand binding to the NOTCH3 receptor mediates transendocytosis of NOTCH3-ADCs into ligand-expressing cells. NOTCH3-ADCs internalize into receptor and ligand cells impartial of signaling and induce cell dying in each cell sorts representing an atypical mechanism of ADC cytotoxicity. Therapy of xenografts with NOTCH3-ADCs results in sustained tumor regressions, outperforms standard-of-care chemotherapy, and permits concentrating on of tumors that overexpress NOTCH3 impartial of signaling inhibition.

Fc-binding antibody-recruiting molecules exploit endogenous antibodies for anti-tumor immune responses

 

Redirecting endogenous antibodies in the bloodstream to tumor cells utilizing artificial molecules is a promising strategy to set off anti-tumor immune responses. Nonetheless, present molecular designs solely allow the usage of a small fraction of endogenous antibodies, limiting the therapeutic potential. Right here, we report Fc-binding antibody-recruiting molecules (Fc-ARMs) as the primary instance addressing this subject. Fc-ARMs are composed of an Fc-binding peptide and a concentrating on ligand, enabling the exploitation of endogenous antibodies via fixed affinity to the Fc area of antibodies, whose sequence is conserved in distinction to the Fab area.

We present that Fc-ARM concentrating on folate receptor-α (FR-α) redirects a clinically used antibody combination to FR-α+ most cancers cells, leading to most cancers cell lysis by pure killer cells in vitro. Fc-ARMs efficiently interacted with antibodies in vivo and amassed in tumors. Moreover, Fc-ARMs recruited antibodies to suppress tumor progress in a mouse mannequin. Thus, Fc-ARMs have the potential to be a novel class of most cancers immunotherapeutic brokers.

Significance of hepatitis B floor antigen, IgM/IgG core antibody and hepatitis B virus DNA in blood donors

 

Introduction: Identification of hepatitis B virus carriers in blood donors is crucial with a view to keep away from transmission of the illness through blood transfusion.

Goal: To find out if blood donors with constructive outcomes for serological markers HBsAg and anti-HBc have been hepatitis B virus DNA carriers.

Strategies: 12,745 samples have been collected from six Ecuadorian blood banks and analyzed for HBsAg, anti-HBc and anti-HBs infectious markers by automated ELISA. All samples that examined constructive for one, two or all three markers have been analyzed with molecular methods to find out the presence of viral DNA.

Outcomes: 27.5 % of the samples that have been reactive for anti-HBc alone and 100 % of these with constructive outcomes for HbsAg and IgM/IgG anti-HBc have been recognized to include hepatitis B virus DNA (p = 0.001).

Conclusions: The choice of an infection markers, in addition to the detection strategies outline the outcomes. Performing two serological and one molecular check is necessary with a view to establish hepatitis B virus carriers and stop its transmission.

Introducción: La identificación de portadores del virus de la hepatitis B en donantes de sangre es imperativo para evitar la transmisión de la enfermedad a través de transfusiones sanguíneas.

Objetivo: Determinar si los donantes de sangre con resultados positivos en los marcadores serológicos HbsAg y anti-HBc eran portadores del ADN del virus de la hepatitis B.

Métodos: Se recolectaron 12 745 muestras de seis bancos de sangre ecuatorianos, las cuales fueron analizadas con pruebas serológicas para identificar marcadores infecciosos de HBsAg, anti-HBc, anti-HBs mediante ELISA automatizada. Todas las muestras positivas para uno, dos o los tres marcadores fueron analizadas con técnica molecular para determinar la presencia del ADN viral.

Resultados: Se identificó que 27.5 % de las muestras reactivas solo a anti-HBc y 100 % de las muestras con resultados positivos de HBsAg/anti-HBc-IgM/IgG presentaron ADN del virus de la hepatitis B (p = 0.001).

Conclusiones: La elección de los marcadores de infección y los métodos de detección definen los resultados. Es importante la realización de dos pruebas serológicas y una molecular para identificar a los portadores del virus de la hepatitis B y evitar su transmisión.

kashbiotech
kashbiotech

Two human antibodies to a meningococcal serogroup B vaccine antigen improve binding of complement Issue H by stabilizing the Issue H binding web site

 

Microbial pathogens bind host complement regulatory proteins to evade the immune system. The bacterial pathogen Neisseria meningitidis, or meningococcus, binds a number of complement regulators, together with human Issue H (FH). FH binding protein (FHbp) is a element of two licensed meningococcal vaccines and in mice FHbp elicits antibodies that inhibit binding of FH to FHbp, which defeat the bacterial evasion mechanism. Nonetheless, people vaccinated with FHbp develop antibodies that improve binding of FH to the micro organism, which might restrict the effectiveness of the vaccines. Within the current research, we present that two vaccine-elicited antibody fragments (Fabs) remoted from completely different human topics improve binding of complement FH to meningococcal FHbp by ELISA.

The 2 Fabs have completely different results on the kinetics of FH binding to immobilized FHbp as measured by floor plasmon resonance. The 1.7- and a couple of.0-Å decision X-ray crystal buildings of the Fabs in complexes with FHbp illustrate that the 2 Fabs bind to comparable epitopes on the amino-terminal area of FHbp, adjoining to the FH binding web site.

Superposition fashions of ternary complexes of every Fab with FHbp and FH present that there’s probably minimal contact between the Fabs and FH. Collectively, the buildings reveal that the Fabs improve binding of FH to FHbp by altering the conformations and mobilities of two loops adjoining to the FH binding web site of FHbp. As well as, the 1.5 Å-resolution construction of one of many remoted Fabs defines the structural rearrangements related to binding to FHbp. The FH-enhancing human Fabs, that are mirrored within the human polyclonal antibody responses, have necessary implications for tuning the effectiveness of FHbp-based vaccines.

 

Anti-Human Integrin alpha 5 Antibody

101-M518 100 µg
EUR 399
Description: Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. They exist as heterodimers consisting of alpha and beta subunits. Some alpha and beta subunits exhibit specificity for one another and may be designated as a VLA (very late antigen) member. Heterodimers often preferentially bind certain cell adhesion molecules, or constituents of the ECM. Although they have no catalytic activity, integrins can be part of multimolecular signaling complexes known as focal adhesions.

Human Integrin alpha-5 ELISA Kit

EK4913 96 tests
EUR 739

Integrin alpha-5

AP84250 1mg
EUR 2640

Integrin alpha-5

AP84313 1mg
EUR 2640

Integrin alpha-5

AP84459 1mg
EUR 2640

Human Integrin Alpha 5 ELISA Kit

E01A5456 96T
EUR 700
Description: ELISA

Human Integrin Alpha 5 ELISA Kit

IHUITGA5KT each
EUR 700
Description: Human Integrin Alpha 5 ELISA Kit

Integrin alpha 5

E8ET1701-58 100ul
EUR 275
Description: Available in various conjugation types.

Integrin alpha-5 Antibody

abx234347-100ug 100 ug
EUR 610.8

Integrin alpha-5 Antibody

abx234347-100g 100 µg
EUR 350

Human Integrin Alpha 5 (ITGa5) Protein

20-abx067272
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  • 100 ug
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Human Integrin Alpha 5 (ITGa5) Protein

abx067272-100g 100 µg
EUR 550

Human Integrin Alpha 5 (ITGa5) Protein

abx067272-10g 10 µg
EUR 212.5

Human Integrin Alpha 5 (ITGa5) Protein

abx067272-50g 50 µg
EUR 387.5

Integrin alpha 5 Antibody

F41992-0.4ML 0.4 ml
EUR 322.15
Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq].

Integrin alpha 5 Antibody

F49220-0.4ML 0.4 ml
EUR 322.15
Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq].

Integrin alpha 5 Antibody

AF5200 200ul
EUR 420

Integrin alpha 5 Antibody

AF5200-100ul 100ul
EUR 280

Integrin alpha 5 Antibody

AF5200-200ul 200ul
EUR 350

Integrin alpha 5 Antibody

49269 100ul
EUR 529

Integrin alpha 5 Antibody

49269-100ul 100ul
EUR 399.6

Integrin alpha 5 Antibody

49269-50ul 50ul
EUR 286.8

Integrin alpha 5 Antibody

R31667 100 ug
EUR 356.15
Description: Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Human Integrin alpha-5(ITGA5) Elisa Kit

EK712764 96 Wells
EUR 0.29

Human Integrin alpha-5(ITGA5) Elisa kit

CSB-E17350h-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5 (ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid (CSF). A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Integrin alpha-5(ITGA5) Elisa kit

1-CSB-E17350h
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  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5(ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid(CSF). Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Integrin alpha-5 (ITGA5) ELISA Kit

RK11332 96T
EUR 280

Human Integrin alpha-5(ITGA5) Elisa kit

YLA4349HU-48T 48T Ask for price

Human Integrin alpha-5(ITGA5) Elisa kit

YLA4349HU-96T 96T Ask for price

Mouse Anti Human Integrin Alpha V Beta 5 Monoclonal Antibody

CABT-49626MH 0.1 mg
EUR 1650

Human Integrin Alpha 5 (ITGa5) CLIA Kit

20-abx492595
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human ITGA5/ Integrin alpha-5 ELISA Kit

E1342Hu 1 Kit
EUR 515
Description: FNRA,CD49 antigen-like family member E,Fibronectin receptor subunit alpha,Integrin alpha-F,VLA-5,CD49e

Human ITGA5(Integrin alpha-5) ELISA Kit

EH2445 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human ITGA5 / Integrin alpha-5 ELISA Kit

E9148h 96T
EUR 736

Human Integrin Alpha 5 (ITGa5) ELISA Kit

DLR-ITGa5-Hu 96T
EUR 443
Description: tissue homogenates, cell lysates or other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit

DLR-ITGa5-Hu-48T 48T
EUR 597.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit

DLR-ITGa5-Hu-96T 96T
EUR 776.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Integrin alpha- 5, ITGA5 ELISA KIT

ELI-07691h 96 Tests
EUR 988.8

Human Integrin Alpha 5 (ITGa5) ELISA Kit

EKN46145-48T 48T
EUR 359.87

Human Integrin Alpha 5 (ITGa5) ELISA Kit

EKN46145-5x96T 5x96T
EUR 2441.98

Human Integrin Alpha 5 (ITGa5) ELISA Kit

EKN46145-96T 96T
EUR 514.1

Human Integrin Alpha 5 (ITGa5) ELISA Kit

EKU05034-48T 48T
EUR 527.66

Human Integrin Alpha 5 (ITGa5) ELISA Kit

EKU05034-5x96T 5x96T
EUR 3580.55

Human Integrin Alpha 5 (ITGa5) ELISA Kit

EKU05034-96T 96T
EUR 753.8

Human Integrin Alpha 5 (ITGa5) ELISA Kit

DL-ITGa5-Hu 96T
EUR 421
Description: tissue homogenates, cell lysates or other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit

EK15036 96Т
EUR 768

Human Integrin Alpha 5 (ITGA5) ELISA Kit

abx571172-96tests 96 tests
EUR 886.8

Human Integrin Alpha 5 (ITGa5) ELISA Kit

20-abx151987
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Integrin Alpha 5 (ITGa5) ELISA Kit

RD-ITGa5-Hu-48T 48T
EUR 432.3
Description: tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit

RD-ITGa5-Hu-48Tests 48 Tests
EUR 600

Human Integrin Alpha 5 (ITGa5) ELISA Kit

RD-ITGa5-Hu-96T 96T
EUR 617.5
Description: tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit

RD-ITGa5-Hu-96Tests 96 Tests
EUR 830.4

Human Integrin Alpha 5 (ITGa5) ELISA Kit

RDR-ITGa5-Hu-48T 48T
EUR 453.92
Description: tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit

RDR-ITGa5-Hu-48Tests 48 Tests
EUR 626.4

Human Integrin Alpha 5 (ITGa5) ELISA Kit

RDR-ITGa5-Hu-96T 96T
EUR 648.38
Description: tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit

RDR-ITGa5-Hu-96Tests 96 Tests
EUR 868.8

Human Integrin Alpha 5(ITGa5) ELISA Kit

QY-E05294 96T
EUR 472.8

Human Integrin Alpha 5 (ITGa5) ELISA Kit

SEB287Hu-10x96wellstestplate 10x96-wells test plate
EUR 5402.92
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit

SEB287Hu-1x48wellstestplate 1x48-wells test plate
EUR 550.13
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit

SEB287Hu-1x96wellstestplate 1x96-wells test plate
EUR 734.46
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit

SEB287Hu-5x96wellstestplate 5x96-wells test plate
EUR 2945.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.

Human Integrin Alpha 5 (ITGa5) ELISA Kit

4-SEB287Hu
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  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Integrin Alpha 5 (ITGa5) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Integrin Alpha-5, ITGA5 GENLISA ELISA

KBH5482 1 x 96 wells
EUR 286

Integrin Alpha 5 (ITGa5) Polyclonal Antibody (Human)

4-PAB287Hu01
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  • 100ul
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  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Integrin Alpha 5 (ITGa5)

Human ITGa5(Integrin Alpha 5) ELISA Kit

ELK3746-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITGa5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITGa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITGa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ITGa5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human ITGa5(Integrin Alpha 5) ELISA Kit

ELK3746-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITGa5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITGa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITGa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ITGa5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human integrin alpha-5 beta-3, His-tag

REC31719-100 0.1
EUR 463.1
Description: Recombinant human integrin alpha-5 beta-3 protein produced in HEK293 with a C-terminal 6x histidine tag.

Human integrin alpha-5 beta-3, His-tag

REC31719-500 0.5
EUR 1622.75
Description: Recombinant human integrin alpha-5 beta-3 protein produced in HEK293 with a C-terminal 6x histidine tag.

Integrin alpha 5 Antibody (ITGA5)

F41992-0.08ML 0.08 ml
EUR 140.25
Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq].

Integrin alpha 5 Antibody (ITGA5)

F49220-0.08ML 0.08 ml
EUR 140.25
Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq].

Integrin Alpha 5 (ITGA5) Antibody

20-abx338742
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  • 100 ug
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  • 20 ug
  • 50 ug

Integrin Alpha 5 (ITGA5) Antibody

20-abx323564
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  • 100 ug
  • 50 ug

Integrin Alpha 5 (ITGA5) Antibody

20-abx325454
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  • 100 ug
  • 50 ug

Integrin Alpha 5 (ITGA5) Antibody

20-abx327245
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  • 100 ug
  • 50 ug

Integrin Alpha 5 (ITGa5) Antibody

20-abx101634
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  • 100 ug
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Integrin Alpha 5 (ITGa5) Antibody

20-abx101635
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  • 100 ug
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Integrin Alpha 5 (ITGa5) Antibody

20-abx129339
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  • 100 ug
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  • 1 mg
  • 200 ug
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Integrin Alpha 5 (ITGa5) Antibody

20-abx179024
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  • 1 mg
  • 200 ug

Integrin Alpha 5 (ITGa5) Antibody

20-abx172956
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  • 1 mg
  • 200 ug

Integrin alpha 5 (CD49e) Antibody

abx216264-100ug 100 ug
EUR 526.8

Integrin Alpha 5 (ITGA5) Antibody

20-abx113185
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  • 150 ul
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Integrin Alpha 5 (ITGA5) Antibody

20-abx006811
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  • 100 ul
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Integrin Alpha 5 (ITGA5) Antibody

abx011678-100ul 100 ul
EUR 493.2

 

Johnny Torres